Optimized CRISPR-RfxCas13d system for RNA targeting in zebrafish embryos

Optimized CRISPR-RfxCas13d system for RNA targeting in zebrafish embryos

Summary: CRISPR-Cas systems have been used to induce DNA mutagenesis for gene function discovery. However, the development of tools to eliminate RNAs provides complementary and unique approaches to disrupt gene expression. Here, we present a workflow to perform specific, efficient, and cost-effectiv...

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Bibliographic Details
Main Authors: Luis Hernandez-Huertas, Gopal Kushawah, Alejandro Diaz-Moscoso, Laura Tomas-Gallardo, Ismael Moreno-Sanchez, Gabriel da Silva Pescador, Ariel A. Bazzini, Miguel A. Moreno-Mateos
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Biotechnology and bioengineering
CRISPR
Developmental biology
Gene Expression
Genetics
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721007644

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http://www.sciencedirect.com/science/article/pii/S2666166721007644

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