Using mass cytometry for the analysis of samples of the human airways

Using mass cytometry for the analysis of samples of the human airways

Mass cytometry (MC) is a powerful method for mapping complex cellular systems at single-cell levels, based on the detection of cellular proteins. Numerous studies have been performed using human blood, but there is a lack of protocols describing the processing and labeling of bronchoalveolar lavage...

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Bibliographic Details
Main Authors: Marianne Rocha-Hasler, Lena Müller, Anja Wagner, Aldine Tu, Victoria Stanek, Nicholas James Campion, Tina Bartosik, Mohammed Zghaebi, Slagjana Stoshikj, Daniela Gompelmann, Andreas Zech, Henrik Mei, Klaus Kratochwill, Andreas Spittler, Marco Idzko, Sven Schneider, Julia Eckl-Dorna
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-12-01
Series:Frontiers in Immunology
Subjects:
mass cytometry
CyTOF
antibody titration
BALF
nasal polyp
tissue digestion
Online Access:https://www.frontiersin.org/articles/10.3389/fimmu.2022.1004583/full

Internet

https://www.frontiersin.org/articles/10.3389/fimmu.2022.1004583/full

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