Investigating the Impact of Sample Preparation on Mass Spectrometry-Based Drug-To-Antibody Ratio Determination for Cysteine- and Lysine-Linked Antibody–Drug Conjugates

Investigating the Impact of Sample Preparation on Mass Spectrometry-Based Drug-To-Antibody Ratio Determination for Cysteine- and Lysine-Linked Antibody–Drug Conjugates

Antibody–drug conjugates (ADCs) are heterogeneous biotherapeutics and differ vastly in their physicochemical properties depending on their design. The number of small drug molecules covalently attached to each antibody molecule is commonly referred to as the drug-to-antibody ratio (DAR). Established...

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Bibliographic Details
Main Authors: Malin Källsten, Rafael Hartmann, Lucia Kovac, Fredrik Lehmann, Sara Bergström Lind, Jonas Bergquist
Format: Article
Language:English
Published: MDPI AG 2020-09-01
Series:Antibodies
Subjects:
antibody–drug conjugate
sample preparation
mass spectrometry
desalting
trastuzumab
disulfide reduction
Online Access:https://www.mdpi.com/2073-4468/9/3/46

Internet

https://www.mdpi.com/2073-4468/9/3/46

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