Lipase catalyzed synthesis of flavor esters in non-aqueous media: Optimization of the yield of pentyl 2-methylpropanoate by statistical analysis
In this study, the synthesis of pentyl 2-methylpropanoate employing a commercial lipase from Candida rugosa was investigated, the emphasis being placed on analyzing the effects of various process conditions on the yield of ester. The response surface methodology (RSM) and five-level-five-factor cent...
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2008-12-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.shd.org.rs/JSCS/Vol73/No12/JSCS-3793.pdf |
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| author | ZORICA KNEZEVIC-JUGOVIC DEJAN BEZBRADICA ZIVANA JAKOVLJEVIC SUZANA BRANKOVIC-DIMITRIJEVIC DUSAN MIJIN |
| author_facet | ZORICA KNEZEVIC-JUGOVIC DEJAN BEZBRADICA ZIVANA JAKOVLJEVIC SUZANA BRANKOVIC-DIMITRIJEVIC DUSAN MIJIN |
| author_sort | ZORICA KNEZEVIC-JUGOVIC |
| collection | DOAJ |
| description | In this study, the synthesis of pentyl 2-methylpropanoate employing a commercial lipase from Candida rugosa was investigated, the emphasis being placed on analyzing the effects of various process conditions on the yield of ester. The response surface methodology (RSM) and five-level-five-factor central composite rotatable design (CCRD) were used to evaluate the effects of variables, namely the initial water content, 0.0–2.0 % (w/v), the reaction temperature, 35–75 °C, the enzyme concentration, 1.0–5.0 g dm-3, the acid/alcohol mole ratio, 1:2–5:2, and the reaction time, 4–48 h, on the yield (%) of ester. The production of pentyl 2-methylpropanoate was optimized and an ester yield response equation was obtained, enabling the prediction of ester yields from known values of the five main factors. It seems that the enzyme concentration, reaction time and acid/alcohol mole ratio predominantly determine the conversion process, while the amount of added water amount had no significant influence on the ester yield. Conversion of around 92 % of the substrate to ester could be realized using a concentration of lipase as low as 4.0 g dm-3 and in a relatively short time (26 h) at 35 °C, when a high substrate mole ratio of 2.5 was used. |
| first_indexed | 2024-12-10T11:51:42Z |
| format | Article |
| id | doaj.art-6d46318c4a8c48929ca55a48cca3aa29 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 |
| language | English |
| last_indexed | 2024-12-10T11:51:42Z |
| publishDate | 2008-12-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-6d46318c4a8c48929ca55a48cca3aa292022-12-22T01:49:54ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51392008-12-01731211391151Lipase catalyzed synthesis of flavor esters in non-aqueous media: Optimization of the yield of pentyl 2-methylpropanoate by statistical analysisZORICA KNEZEVIC-JUGOVICDEJAN BEZBRADICAZIVANA JAKOVLJEVICSUZANA BRANKOVIC-DIMITRIJEVICDUSAN MIJINIn this study, the synthesis of pentyl 2-methylpropanoate employing a commercial lipase from Candida rugosa was investigated, the emphasis being placed on analyzing the effects of various process conditions on the yield of ester. The response surface methodology (RSM) and five-level-five-factor central composite rotatable design (CCRD) were used to evaluate the effects of variables, namely the initial water content, 0.0–2.0 % (w/v), the reaction temperature, 35–75 °C, the enzyme concentration, 1.0–5.0 g dm-3, the acid/alcohol mole ratio, 1:2–5:2, and the reaction time, 4–48 h, on the yield (%) of ester. The production of pentyl 2-methylpropanoate was optimized and an ester yield response equation was obtained, enabling the prediction of ester yields from known values of the five main factors. It seems that the enzyme concentration, reaction time and acid/alcohol mole ratio predominantly determine the conversion process, while the amount of added water amount had no significant influence on the ester yield. Conversion of around 92 % of the substrate to ester could be realized using a concentration of lipase as low as 4.0 g dm-3 and in a relatively short time (26 h) at 35 °C, when a high substrate mole ratio of 2.5 was used.http://www.shd.org.rs/JSCS/Vol73/No12/JSCS-3793.pdffactorial designsurface response analysisCandida rugosa lipaseflavor estersesterification |
| spellingShingle | ZORICA KNEZEVIC-JUGOVIC DEJAN BEZBRADICA ZIVANA JAKOVLJEVIC SUZANA BRANKOVIC-DIMITRIJEVIC DUSAN MIJIN Lipase catalyzed synthesis of flavor esters in non-aqueous media: Optimization of the yield of pentyl 2-methylpropanoate by statistical analysis Journal of the Serbian Chemical Society factorial design surface response analysis Candida rugosa lipase flavor esters esterification |
| title | Lipase catalyzed synthesis of flavor esters in non-aqueous media: Optimization of the yield of pentyl 2-methylpropanoate by statistical analysis |
| title_full | Lipase catalyzed synthesis of flavor esters in non-aqueous media: Optimization of the yield of pentyl 2-methylpropanoate by statistical analysis |
| title_fullStr | Lipase catalyzed synthesis of flavor esters in non-aqueous media: Optimization of the yield of pentyl 2-methylpropanoate by statistical analysis |
| title_full_unstemmed | Lipase catalyzed synthesis of flavor esters in non-aqueous media: Optimization of the yield of pentyl 2-methylpropanoate by statistical analysis |
| title_short | Lipase catalyzed synthesis of flavor esters in non-aqueous media: Optimization of the yield of pentyl 2-methylpropanoate by statistical analysis |
| title_sort | lipase catalyzed synthesis of flavor esters in non aqueous media optimization of the yield of pentyl 2 methylpropanoate by statistical analysis |
| topic | factorial design surface response analysis Candida rugosa lipase flavor esters esterification |
| url | http://www.shd.org.rs/JSCS/Vol73/No12/JSCS-3793.pdf |
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