Restriction enzyme digestion of host DNA enhances universal detection of parasitic pathogens in blood via targeted amplicon deep sequencing

Restriction enzyme digestion of host DNA enhances universal detection of parasitic pathogens in blood via targeted amplicon deep sequencing

Abstract Background Targeted amplicon deep sequencing (TADS) of the 16S rRNA gene is commonly used to explore and characterize bacterial microbiomes. Meanwhile, attempts to apply TADS to the detection and characterization of entire parasitic communities have been hampered since conserved regions of...

Full description

Bibliographic Details
Main Authors: Briana R. Flaherty, Eldin Talundzic, Joel Barratt, Kristine J. Kines, Christian Olsen, Meredith Lane, Mili Sheth, Richard S. Bradbury
Format: Article
Language:English
Published: BMC 2018-09-01
Series:Microbiome
Subjects:
Molecular parasitology
Amplicon sequencing
Blood microbiota
Parasite biodiversity
Online Access:http://link.springer.com/article/10.1186/s40168-018-0540-2

Internet

http://link.springer.com/article/10.1186/s40168-018-0540-2

Similar Items