Molecular Cloning, Characterization, and Mutagenesis of a pel Gene from Pseudomonas syringae pv. lachrymans Encoding a Member of the Erwinia chrysanthemi PelADE Family of Pectate Lyases

The pelS gene from Pseudomonas syringae pv. lachrymans 859 was cloned by heterologous expression in nonpectolytic P. syringae pv. syringae BUVS1, using genomic DNA libraries constructed with two novel broad-host-range cosmid vectors, pCPP34 and pCPP47. Screening of P. syringae pv. syringae transconj...

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Bibliographic Details
Main Authors: David W. Bauer, Alan Collmer
Format: Article
Language:English
Published: The American Phytopathological Society 1997-04-01
Series:Molecular Plant-Microbe Interactions
Subjects:
Online Access:https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.3.369