Autophagy-prominent cell clusters among human lens epithelial cells: integrated single-cell RNA-sequencing analysis
Abstract Background Autophagy is an important process that maintains the quality of intracellular proteins and organelles. There is extensive evidence that autophagy has an important role in the lens. Human lens epithelial cells (HLECs) play a key role in the internal homeostasis of the lens. HLEC s...
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | BMC Ophthalmology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12886-023-02910-8 |
| _version_ | 1797841148283191296 |
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| author | Jiasheng Liu Mengchao Zhu Yitong Xu Mengdi Zhang Haisen Sun Yaqi Wang Qingwen Yang Jin Li |
| author_facet | Jiasheng Liu Mengchao Zhu Yitong Xu Mengdi Zhang Haisen Sun Yaqi Wang Qingwen Yang Jin Li |
| author_sort | Jiasheng Liu |
| collection | DOAJ |
| description | Abstract Background Autophagy is an important process that maintains the quality of intracellular proteins and organelles. There is extensive evidence that autophagy has an important role in the lens. Human lens epithelial cells (HLECs) play a key role in the internal homeostasis of the lens. HLEC subtypes have been identified, but autophagy-prominent cell clusters among HLECs have not been characterized. Purpose To explore the existence of autophagy-prominent cell clusters in HLECs. Methods Three donated lenses (HLECs from two whole lenses and HLECs from one lens without the anterior central 6-mm zone) were used for single-cell RNA sequencing (scRNA-seq). AUCell and AddModuleScore analysis were used to identify potential autophagy-prominent cell clusters. Transmission electron microscopy (TEM) was used to confirm the results. Results High-quality transcripts from 18,120 cells were acquired by scRNA-seq of the two intact lenses. Unsupervised clustering classified the cells into four clusters. AUCell and AddModuleScore analysis revealed cluster 1 is autophagy-prominent. scRNA-seq analysis of HLECs from the lens capsule lacking the central zone confirmed the cluster 1 HLECs was located in the central capsule zone. The TEM result showed that greater autophagy activity was observed in the HLECs in central capsule zone, which further supported the above conclusions based on scRNA-seq analysis that autophagy was prominent in the central zone where the cluster 1 HLECs located. Conclusions We identified an autophagy-prominent cell cluster among HLECs and revealed that it was localized in the central zone of the lens capsule. Our findings will aid investigations of autophagy in HLECs and provide insights to guide related research. |
| first_indexed | 2024-04-09T16:26:22Z |
| format | Article |
| id | doaj.art-6deb7687339649659bf94d464d75f473 |
| institution | Directory Open Access Journal |
| issn | 1471-2415 |
| language | English |
| last_indexed | 2024-04-09T16:26:22Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Ophthalmology |
| spelling | doaj.art-6deb7687339649659bf94d464d75f4732023-04-23T11:12:13ZengBMCBMC Ophthalmology1471-24152023-04-012311710.1186/s12886-023-02910-8Autophagy-prominent cell clusters among human lens epithelial cells: integrated single-cell RNA-sequencing analysisJiasheng Liu0Mengchao Zhu1Yitong Xu2Mengdi Zhang3Haisen Sun4Yaqi Wang5Qingwen Yang6Jin Li7Department of Cataract, Eye Hospital of Wenzhou Medical UniversityDepartment of Cataract, Eye Hospital of Wenzhou Medical UniversityDepartment of Cataract, Eye Hospital of Wenzhou Medical UniversityDepartment of Cataract, Eye Hospital of Wenzhou Medical UniversityDepartment of Cataract, Eye Hospital of Wenzhou Medical UniversityDepartment of Cataract, Eye Hospital of Wenzhou Medical UniversityDepartment of Cataract, Eye Hospital of Wenzhou Medical UniversityDepartment of Cataract, Eye Hospital of Wenzhou Medical UniversityAbstract Background Autophagy is an important process that maintains the quality of intracellular proteins and organelles. There is extensive evidence that autophagy has an important role in the lens. Human lens epithelial cells (HLECs) play a key role in the internal homeostasis of the lens. HLEC subtypes have been identified, but autophagy-prominent cell clusters among HLECs have not been characterized. Purpose To explore the existence of autophagy-prominent cell clusters in HLECs. Methods Three donated lenses (HLECs from two whole lenses and HLECs from one lens without the anterior central 6-mm zone) were used for single-cell RNA sequencing (scRNA-seq). AUCell and AddModuleScore analysis were used to identify potential autophagy-prominent cell clusters. Transmission electron microscopy (TEM) was used to confirm the results. Results High-quality transcripts from 18,120 cells were acquired by scRNA-seq of the two intact lenses. Unsupervised clustering classified the cells into four clusters. AUCell and AddModuleScore analysis revealed cluster 1 is autophagy-prominent. scRNA-seq analysis of HLECs from the lens capsule lacking the central zone confirmed the cluster 1 HLECs was located in the central capsule zone. The TEM result showed that greater autophagy activity was observed in the HLECs in central capsule zone, which further supported the above conclusions based on scRNA-seq analysis that autophagy was prominent in the central zone where the cluster 1 HLECs located. Conclusions We identified an autophagy-prominent cell cluster among HLECs and revealed that it was localized in the central zone of the lens capsule. Our findings will aid investigations of autophagy in HLECs and provide insights to guide related research.https://doi.org/10.1186/s12886-023-02910-8Human lens epithelial cellsAutophagySingle-cell RNA-sequencingTransmission electron microscopy |
| spellingShingle | Jiasheng Liu Mengchao Zhu Yitong Xu Mengdi Zhang Haisen Sun Yaqi Wang Qingwen Yang Jin Li Autophagy-prominent cell clusters among human lens epithelial cells: integrated single-cell RNA-sequencing analysis BMC Ophthalmology Human lens epithelial cells Autophagy Single-cell RNA-sequencing Transmission electron microscopy |
| title | Autophagy-prominent cell clusters among human lens epithelial cells: integrated single-cell RNA-sequencing analysis |
| title_full | Autophagy-prominent cell clusters among human lens epithelial cells: integrated single-cell RNA-sequencing analysis |
| title_fullStr | Autophagy-prominent cell clusters among human lens epithelial cells: integrated single-cell RNA-sequencing analysis |
| title_full_unstemmed | Autophagy-prominent cell clusters among human lens epithelial cells: integrated single-cell RNA-sequencing analysis |
| title_short | Autophagy-prominent cell clusters among human lens epithelial cells: integrated single-cell RNA-sequencing analysis |
| title_sort | autophagy prominent cell clusters among human lens epithelial cells integrated single cell rna sequencing analysis |
| topic | Human lens epithelial cells Autophagy Single-cell RNA-sequencing Transmission electron microscopy |
| url | https://doi.org/10.1186/s12886-023-02910-8 |
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