A detailed methodology for a three-dimensional, self-structuring bone model that supports the differentiation of osteoblasts towards osteocytes and the production of a complex collagen-rich mineralised matrix [version 3; peer review: 1 approved, 3 approved with reservations]

A detailed methodology for a three-dimensional, self-structuring bone model that supports the differentiation of osteoblasts towards osteocytes and the production of a complex collagen-rich mineralised matrix [version 3; peer review: 1 approved, 3 approved with reservations]

Background There are insufficient in vitro bone models that accommodate long-term culture of osteoblasts and support their differentiation to osteocytes. The increased demand for effective therapies for bone diseases, and the ethical requirement to replace animals in research, warrants the developme...

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Bibliographic Details
Main Authors: Amy J Naylor, Simon W Jones, Liam M Grover, Thomas Nicholson, James Edwards, Kieran Patrick, Jonathan W Lewis, Hannah C Lamont, Kathryn Frost, Binal Patel, Miruna Chipara, Laurence A Hill, Georgiana Neag, Melissa Finlay
Format: Article
Language:English
Published: F1000 Research Ltd 2024-07-01
Series:F1000Research
Subjects:
Osteocyte
bone
animal reduction
3D model
in vitro culture
eng
Online Access:https://f1000research.com/articles/12-357/v3

Internet

https://f1000research.com/articles/12-357/v3

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