Protocol for optimized dissociation of human scalp tissue for hair follicle transcriptomics by scRNA-seq

Protocol for optimized dissociation of human scalp tissue for hair follicle transcriptomics by scRNA-seq

Summary: Single-cell RNA sequencing (scRNA-seq) is a powerful tool for studying transcriptomics. Here, we present an optimized protocol for dissociating human scalp tissue and acquiring high-quality single-cell suspension for scRNA-seq to study transcriptomics of human hair follicles. We describe st...

Full description

Bibliographic Details
Main Authors: Zishuo Yuan, Junjie Chen, Yanwen Xu, Zhentao Zhou, Pengfei Cai, Xiaoyu Wei, Huiwen Zheng, Jufang Zhang, Yue Yuan, Chuanyu Liu
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell-based Assays
Genetics
Genomics
Molecular Biology
Single Cell
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724000133

Internet

http://www.sciencedirect.com/science/article/pii/S2666166724000133

Similar Items