Protocol for optimized dissociation of human scalp tissue for hair follicle transcriptomics by scRNA-seq
Summary: Single-cell RNA sequencing (scRNA-seq) is a powerful tool for studying transcriptomics. Here, we present an optimized protocol for dissociating human scalp tissue and acquiring high-quality single-cell suspension for scRNA-seq to study transcriptomics of human hair follicles. We describe st...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724000133 |
| _version_ | 1797323773783834624 |
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| author | Zishuo Yuan Junjie Chen Yanwen Xu Zhentao Zhou Pengfei Cai Xiaoyu Wei Huiwen Zheng Jufang Zhang Yue Yuan Chuanyu Liu |
| author_facet | Zishuo Yuan Junjie Chen Yanwen Xu Zhentao Zhou Pengfei Cai Xiaoyu Wei Huiwen Zheng Jufang Zhang Yue Yuan Chuanyu Liu |
| author_sort | Zishuo Yuan |
| collection | DOAJ |
| description | Summary: Single-cell RNA sequencing (scRNA-seq) is a powerful tool for studying transcriptomics. Here, we present an optimized protocol for dissociating human scalp tissue and acquiring high-quality single-cell suspension for scRNA-seq to study transcriptomics of human hair follicles. We describe steps for human scalp tissue cleaning, subcutaneous fat removal, mechanical mincing, and enzymatic digestion. We then detail procedures for cleaning, resuspending, a cell viability assay, and library construction. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-08T05:33:59Z |
| format | Article |
| id | doaj.art-6e0f3edcd7d34ab581fea5d99cd6fa74 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-08T05:33:59Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-6e0f3edcd7d34ab581fea5d99cd6fa742024-02-06T04:12:49ZengElsevierSTAR Protocols2666-16672024-03-0151102848Protocol for optimized dissociation of human scalp tissue for hair follicle transcriptomics by scRNA-seqZishuo Yuan0Junjie Chen1Yanwen Xu2Zhentao Zhou3Pengfei Cai4Xiaoyu Wei5Huiwen Zheng6Jufang Zhang7Yue Yuan8Chuanyu Liu9BGI Research, Hangzhou 310030, China; BGI Research, Shenzhen 518083, ChinaBGI Research, Hangzhou 310030, ChinaDepartment of Plastic Surgery, Hangzhou First People’s Hospital, Hangzhou Zhejiang 310006, ChinaDepartment of Plastic Surgery, Hangzhou First People’s Hospital, Hangzhou Zhejiang 310006, ChinaBGI Research, Hangzhou 310030, China; BGI Research, Shenzhen 518083, ChinaBGI Research, Hangzhou 310030, China; BGI Research, Shenzhen 518083, ChinaBGI Research, Hangzhou 310030, ChinaDepartment of Plastic Surgery, Hangzhou First People’s Hospital, Hangzhou Zhejiang 310006, ChinaBGI Research, Hangzhou 310030, China; BGI Research, Shenzhen 518083, China; CorrespondenceBGI Research, Hangzhou 310030, China; BGI Research, Shenzhen 518083, China; CorrespondenceSummary: Single-cell RNA sequencing (scRNA-seq) is a powerful tool for studying transcriptomics. Here, we present an optimized protocol for dissociating human scalp tissue and acquiring high-quality single-cell suspension for scRNA-seq to study transcriptomics of human hair follicles. We describe steps for human scalp tissue cleaning, subcutaneous fat removal, mechanical mincing, and enzymatic digestion. We then detail procedures for cleaning, resuspending, a cell viability assay, and library construction. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724000133Cell BiologyCell-based AssaysGeneticsGenomicsMolecular BiologySingle Cell |
| spellingShingle | Zishuo Yuan Junjie Chen Yanwen Xu Zhentao Zhou Pengfei Cai Xiaoyu Wei Huiwen Zheng Jufang Zhang Yue Yuan Chuanyu Liu Protocol for optimized dissociation of human scalp tissue for hair follicle transcriptomics by scRNA-seq STAR Protocols Cell Biology Cell-based Assays Genetics Genomics Molecular Biology Single Cell |
| title | Protocol for optimized dissociation of human scalp tissue for hair follicle transcriptomics by scRNA-seq |
| title_full | Protocol for optimized dissociation of human scalp tissue for hair follicle transcriptomics by scRNA-seq |
| title_fullStr | Protocol for optimized dissociation of human scalp tissue for hair follicle transcriptomics by scRNA-seq |
| title_full_unstemmed | Protocol for optimized dissociation of human scalp tissue for hair follicle transcriptomics by scRNA-seq |
| title_short | Protocol for optimized dissociation of human scalp tissue for hair follicle transcriptomics by scRNA-seq |
| title_sort | protocol for optimized dissociation of human scalp tissue for hair follicle transcriptomics by scrna seq |
| topic | Cell Biology Cell-based Assays Genetics Genomics Molecular Biology Single Cell |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724000133 |
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