Influences of Microwave-Assisted Extraction Parameters on Antioxidant Activity of the Extract from <i>Akebia trifoliata</i> Peels
<i>Akebia trifoliata</i> is a fruit with rich nutritional properties, and its peel is produced as a by-product. In this research, we investigated the influences of microwave-assisted extraction parameters on antioxidant activity of the extract from <i>Akebia trifoliata</i> pe...
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MDPI AG
2021-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/6/1432 |
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| author | Min Luo Dan-Dan Zhou Ao Shang Ren-You Gan Hua-Bin Li |
| author_facet | Min Luo Dan-Dan Zhou Ao Shang Ren-You Gan Hua-Bin Li |
| author_sort | Min Luo |
| collection | DOAJ |
| description | <i>Akebia trifoliata</i> is a fruit with rich nutritional properties, and its peel is produced as a by-product. In this research, we investigated the influences of microwave-assisted extraction parameters on antioxidant activity of the extract from <i>Akebia trifoliata</i> peels, and the ferric-reducing antioxidant power (FRAP) and Trolox equivalent antioxidant capacity (TEAC) as well as total phenolic contents (TPC) were used to optimize extraction parameters. The influences of ethanol concentration, microwave power and solvent-to-material ratio, as well as extraction temperature and time on TPC, FRAP and TEAC values, were assessed using single-factor tests. Three parameters with obvious effects on antioxidant capacity were selected to further investigate their interactions by response surface methodology. The optimal extraction parameters of natural antioxidants from <i>Akebia trifoliata</i> peels were ethanol concentration, 49.61% (<i>v</i>/<i>v</i>); solvent-to-material ratio, 32.59:1 mL/g; extraction time, 39.31 min; microwave power, 500 W; and extraction temperature, 50 °C. Under optimal conditions, the FRAP, TEAC and TPC values of <i>Akebia trifoliata</i> peel extracts were 351.86 ± 9.47 µM Fe(II)/g dry weight (DW), 191.12 ± 3.53 µM Trolox/g DW and 32.67 ± 0.90 mg gallic acid equivalent (GAE)/g DW, respectively. Furthermore, the main bioactive compounds (chlorogenic acid, rutin and ellagic acid) in the extract were determined by high-performance liquid chromatography. The results are useful for the full utilization of the by-product from <i>Akebia trifoliate</i> fruit. |
| first_indexed | 2024-03-10T10:13:42Z |
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| last_indexed | 2024-03-10T10:13:42Z |
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| series | Foods |
| spelling | doaj.art-6e1af4672abb474696d4af1bfe45fef32023-11-22T00:59:57ZengMDPI AGFoods2304-81582021-06-01106143210.3390/foods10061432Influences of Microwave-Assisted Extraction Parameters on Antioxidant Activity of the Extract from <i>Akebia trifoliata</i> PeelsMin Luo0Dan-Dan Zhou1Ao Shang2Ren-You Gan3Hua-Bin Li4Guangdong Provincial Key Laboratory of Food, Nutrition and Health, Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, ChinaGuangdong Provincial Key Laboratory of Food, Nutrition and Health, Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, ChinaGuangdong Provincial Key Laboratory of Food, Nutrition and Health, Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, ChinaResearch Center for Plants and Human Health, Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu 610213, ChinaGuangdong Provincial Key Laboratory of Food, Nutrition and Health, Department of Nutrition, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China<i>Akebia trifoliata</i> is a fruit with rich nutritional properties, and its peel is produced as a by-product. In this research, we investigated the influences of microwave-assisted extraction parameters on antioxidant activity of the extract from <i>Akebia trifoliata</i> peels, and the ferric-reducing antioxidant power (FRAP) and Trolox equivalent antioxidant capacity (TEAC) as well as total phenolic contents (TPC) were used to optimize extraction parameters. The influences of ethanol concentration, microwave power and solvent-to-material ratio, as well as extraction temperature and time on TPC, FRAP and TEAC values, were assessed using single-factor tests. Three parameters with obvious effects on antioxidant capacity were selected to further investigate their interactions by response surface methodology. The optimal extraction parameters of natural antioxidants from <i>Akebia trifoliata</i> peels were ethanol concentration, 49.61% (<i>v</i>/<i>v</i>); solvent-to-material ratio, 32.59:1 mL/g; extraction time, 39.31 min; microwave power, 500 W; and extraction temperature, 50 °C. Under optimal conditions, the FRAP, TEAC and TPC values of <i>Akebia trifoliata</i> peel extracts were 351.86 ± 9.47 µM Fe(II)/g dry weight (DW), 191.12 ± 3.53 µM Trolox/g DW and 32.67 ± 0.90 mg gallic acid equivalent (GAE)/g DW, respectively. Furthermore, the main bioactive compounds (chlorogenic acid, rutin and ellagic acid) in the extract were determined by high-performance liquid chromatography. The results are useful for the full utilization of the by-product from <i>Akebia trifoliate</i> fruit.https://www.mdpi.com/2304-8158/10/6/1432microwave-assisted extraction<i>Akebia trifoliate</i>peelresponse surface methodologyantioxidant capacitytotal phenolic content |
| spellingShingle | Min Luo Dan-Dan Zhou Ao Shang Ren-You Gan Hua-Bin Li Influences of Microwave-Assisted Extraction Parameters on Antioxidant Activity of the Extract from <i>Akebia trifoliata</i> Peels Foods microwave-assisted extraction <i>Akebia trifoliate</i> peel response surface methodology antioxidant capacity total phenolic content |
| title | Influences of Microwave-Assisted Extraction Parameters on Antioxidant Activity of the Extract from <i>Akebia trifoliata</i> Peels |
| title_full | Influences of Microwave-Assisted Extraction Parameters on Antioxidant Activity of the Extract from <i>Akebia trifoliata</i> Peels |
| title_fullStr | Influences of Microwave-Assisted Extraction Parameters on Antioxidant Activity of the Extract from <i>Akebia trifoliata</i> Peels |
| title_full_unstemmed | Influences of Microwave-Assisted Extraction Parameters on Antioxidant Activity of the Extract from <i>Akebia trifoliata</i> Peels |
| title_short | Influences of Microwave-Assisted Extraction Parameters on Antioxidant Activity of the Extract from <i>Akebia trifoliata</i> Peels |
| title_sort | influences of microwave assisted extraction parameters on antioxidant activity of the extract from i akebia trifoliata i peels |
| topic | microwave-assisted extraction <i>Akebia trifoliate</i> peel response surface methodology antioxidant capacity total phenolic content |
| url | https://www.mdpi.com/2304-8158/10/6/1432 |
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