Construction and characterization of a bacterial artificial chromosome library for Gossypium mustelinum.

A bacterial artificial chromosome (BAC) library for G. mustelinum Miers ex G. Watt (AD4) was constructed. Intact nuclei from G. mustelinum (AD4) were used to isolate high molecular weight DNA, which was partially cleaved with Hind III and cloned into pSMART BAC (Hind III) vectors. The BAC library co...

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Bibliographic Details
Main Authors: Yuling Liu, Baohong Zhang, Xinpeng Wen, Shulin Zhang, Yangyang Wei, Quanwei Lu, Zhen Liu, Kunbo Wang, Fang Liu, Renhai Peng
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5957370?pdf=render