Kurarinone Inhibits HCoV-OC43 Infection by Impairing the Virus-Induced Autophagic Flux in MRC-5 Human Lung Cells
Kurarinone is a prenylated flavonone isolated from the roots of <i>Sophora flavescens</i>. Among its known functions, kurarinone has both anti-apoptotic and anti-inflammatory properties. Coronaviruses (CoVs), including HCoV-OC43, SARS-CoV, MERS-CoV, and SARS-CoV-2, are the causative agen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/9/7/2230 |
| _version_ | 1797562471561560064 |
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| author | Jung Sun Min Dong Eon Kim Young-Hee Jin Sunoh Kwon |
| author_facet | Jung Sun Min Dong Eon Kim Young-Hee Jin Sunoh Kwon |
| author_sort | Jung Sun Min |
| collection | DOAJ |
| description | Kurarinone is a prenylated flavonone isolated from the roots of <i>Sophora flavescens</i>. Among its known functions, kurarinone has both anti-apoptotic and anti-inflammatory properties. Coronaviruses (CoVs), including HCoV-OC43, SARS-CoV, MERS-CoV, and SARS-CoV-2, are the causative agents of respiratory virus infections that range in severity from the common cold to severe pneumonia. There are currently no effective treatments for coronavirus-associated diseases. In this report, we examined the anti-viral impact of kurarinone against infection with the human coronavirus, HCoV-OC43. We found that kurarinone inhibited HCoV-OC43 infection in human lung fibroblast MRC-5 cells in a dose-dependent manner with an IC<sub>50</sub> of 3.458 ± 0.101 µM. Kurarinone inhibited the virus-induced cytopathic effect, as well as extracellular and intracellular viral RNA and viral protein expression. Time-of-addition experiments suggested that kurarinone acted at an early stage of virus infection. Finally, we found that HCoV-OC43 infection increased the autophagic flux in MRC-5 cells; kurarinone inhibited viral replication via its capacity to impair the virus-induced autophagic flux. As such, we suggest that kurarinone may be a useful therapeutic for the treatment of diseases associated with coronavirus infection. |
| first_indexed | 2024-03-10T18:29:40Z |
| format | Article |
| id | doaj.art-6e45ceddad504c20bbaf7a1c95e6ccdd |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T18:29:40Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-6e45ceddad504c20bbaf7a1c95e6ccdd2023-11-20T06:43:22ZengMDPI AGJournal of Clinical Medicine2077-03832020-07-0197223010.3390/jcm9072230Kurarinone Inhibits HCoV-OC43 Infection by Impairing the Virus-Induced Autophagic Flux in MRC-5 Human Lung CellsJung Sun Min0Dong Eon Kim1Young-Hee Jin2Sunoh Kwon3Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaHerbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaCenter for Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, KoreaHerbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaKurarinone is a prenylated flavonone isolated from the roots of <i>Sophora flavescens</i>. Among its known functions, kurarinone has both anti-apoptotic and anti-inflammatory properties. Coronaviruses (CoVs), including HCoV-OC43, SARS-CoV, MERS-CoV, and SARS-CoV-2, are the causative agents of respiratory virus infections that range in severity from the common cold to severe pneumonia. There are currently no effective treatments for coronavirus-associated diseases. In this report, we examined the anti-viral impact of kurarinone against infection with the human coronavirus, HCoV-OC43. We found that kurarinone inhibited HCoV-OC43 infection in human lung fibroblast MRC-5 cells in a dose-dependent manner with an IC<sub>50</sub> of 3.458 ± 0.101 µM. Kurarinone inhibited the virus-induced cytopathic effect, as well as extracellular and intracellular viral RNA and viral protein expression. Time-of-addition experiments suggested that kurarinone acted at an early stage of virus infection. Finally, we found that HCoV-OC43 infection increased the autophagic flux in MRC-5 cells; kurarinone inhibited viral replication via its capacity to impair the virus-induced autophagic flux. As such, we suggest that kurarinone may be a useful therapeutic for the treatment of diseases associated with coronavirus infection.https://www.mdpi.com/2077-0383/9/7/2230kurarinonecoronavirusHCoV-OC43autophagyinfectionMRC-5 cell |
| spellingShingle | Jung Sun Min Dong Eon Kim Young-Hee Jin Sunoh Kwon Kurarinone Inhibits HCoV-OC43 Infection by Impairing the Virus-Induced Autophagic Flux in MRC-5 Human Lung Cells Journal of Clinical Medicine kurarinone coronavirus HCoV-OC43 autophagy infection MRC-5 cell |
| title | Kurarinone Inhibits HCoV-OC43 Infection by Impairing the Virus-Induced Autophagic Flux in MRC-5 Human Lung Cells |
| title_full | Kurarinone Inhibits HCoV-OC43 Infection by Impairing the Virus-Induced Autophagic Flux in MRC-5 Human Lung Cells |
| title_fullStr | Kurarinone Inhibits HCoV-OC43 Infection by Impairing the Virus-Induced Autophagic Flux in MRC-5 Human Lung Cells |
| title_full_unstemmed | Kurarinone Inhibits HCoV-OC43 Infection by Impairing the Virus-Induced Autophagic Flux in MRC-5 Human Lung Cells |
| title_short | Kurarinone Inhibits HCoV-OC43 Infection by Impairing the Virus-Induced Autophagic Flux in MRC-5 Human Lung Cells |
| title_sort | kurarinone inhibits hcov oc43 infection by impairing the virus induced autophagic flux in mrc 5 human lung cells |
| topic | kurarinone coronavirus HCoV-OC43 autophagy infection MRC-5 cell |
| url | https://www.mdpi.com/2077-0383/9/7/2230 |
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