CRISPR Interference Efficiently Silences Latent and Lytic Viral Genes in Kaposi’s Sarcoma-Associated Herpesvirus-Infected Cells

CRISPR Interference Efficiently Silences Latent and Lytic Viral Genes in Kaposi’s Sarcoma-Associated Herpesvirus-Infected Cells

Uncovering viral gene functions requires the modulation of gene expression through overexpression or loss-of-function. CRISPR interference (CRISPRi), a modification of the CRISPR-Cas9 gene editing technology, allows specific and efficient transcriptional silencing without genetic ablation. CRISPRi h...

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Bibliographic Details
Main Authors: Kevin Brackett, Ameera Mungale, Mary Lopez-Isidro, Duncan A. Proctor, Guillermo Najarro, Carolina Arias
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Viruses
Subjects:
KSHV
CRISPR-interference
dCas9-KRAB
Kaposi’s sarcoma-associated herpesvirus
gene expression
gene silencing
Online Access:https://www.mdpi.com/1999-4915/13/5/783

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https://www.mdpi.com/1999-4915/13/5/783

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