Reference genes for the developing mouse lung under consideration of biological, technical and experimental confounders

Abstract For gene expression analysis, the raw data obtained from RT-qPCR are preferably normalized to reference genes, which should be constantly expressed regardless of experimental conditions. Selection of reference genes is particularly challenging for the developing lung because of the complex...

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Bibliographic Details
Main Authors: H. Shin, R. E. Morty, J. M. Sucre, N. M. Negretti, M. Markmann, H. Hossain, S. Krauss-Etschmann, S. Dehmel, A. Hilgendorff
Format: Article
Language:English
Published: Nature Portfolio 2022-10-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-022-19071-1