Restored and Enhanced Memory T Cell Immunity in Rheumatoid Arthritis After TNFα Blocker Treatment
TNFα inhibitors have shaped the landscape of rheumatoid arthritis (RA) therapy with high clinical efficiency. However, their impact on T cell recall responses is not well-elucidated. We aimed to analyze the immune profiles of memory T cells in RA patients undergoing TNFα inhibitor Golimumab (GM) tre...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.00887/full |
| _version_ | 1811240077338083328 |
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| author | Asma Khanniche Asma Khanniche Ling Zhou Bin Jiang Jing Song Yanhua Jin Jian Yin Shujun Wang Ping Ji Hao Shen Ying Wang Huji Xu Huji Xu Huji Xu |
| author_facet | Asma Khanniche Asma Khanniche Ling Zhou Bin Jiang Jing Song Yanhua Jin Jian Yin Shujun Wang Ping Ji Hao Shen Ying Wang Huji Xu Huji Xu Huji Xu |
| author_sort | Asma Khanniche |
| collection | DOAJ |
| description | TNFα inhibitors have shaped the landscape of rheumatoid arthritis (RA) therapy with high clinical efficiency. However, their impact on T cell recall responses is not well-elucidated. We aimed to analyze the immune profiles of memory T cells in RA patients undergoing TNFα inhibitor Golimumab (GM) treatment. Frequencies of peripheral T cell subsets and cytokine expression profiles in memory T cells (TM) upon PMA/Ionomycine stimulation were determined by flow cytometry. Antigen-specific CD8 T cell immunity was analyzed through stimulating PBMCs with CMV-EBV-Flu (CEF) viral peptide pool and subsequent intracellular IFNγ staining. Both peripheral CD8 and CD4 T cells from GM treated patients had a shift pattern characterized by an enlarged effector TM and a reduced central TM cell population when compared to GM untreated group. An increase in the frequencies of TNFα+, IL-2+, and IL-17+ CD8 TM cells was observed whereas only TNFα+CD4 TM cells increased in GM treated patients. Moreover, GM treated patients contained more peripheral IFNγ-producing CD8 T cells specific to CEF viral peptides. Together, these results show a distinct T cell subset pattern and enhanced memory T cell immunity upon GM treatment, suggesting an immunoregulatory effect of TNF inhibitor Golimumab on peripheral memory T cell responses. |
| first_indexed | 2024-04-12T13:12:58Z |
| format | Article |
| id | doaj.art-6e6fa17094b448c8a2eb781435cb471a |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-12T13:12:58Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-6e6fa17094b448c8a2eb781435cb471a2022-12-22T03:31:47ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-04-011010.3389/fimmu.2019.00887437341Restored and Enhanced Memory T Cell Immunity in Rheumatoid Arthritis After TNFα Blocker TreatmentAsma Khanniche0Asma Khanniche1Ling Zhou2Bin Jiang3Jing Song4Yanhua Jin5Jian Yin6Shujun Wang7Ping Ji8Hao Shen9Ying Wang10Huji Xu11Huji Xu12Huji Xu13Shanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, ChinaDepartment of Rheumatology and Immunology, Shanghai Chang Zheng Hospital, Second Military Medical University, Shanghai, ChinaDepartment of Rheumatology, Renji Hospital, Shanghai, ChinaDepartment of Rheumatology and Immunology, Shanghai Chang Zheng Hospital, Second Military Medical University, Shanghai, ChinaDepartment of Rheumatology and Immunology, Shanghai Chang Zheng Hospital, Second Military Medical University, Shanghai, ChinaDepartment of Rheumatology and Immunology, Shanghai Chang Zheng Hospital, Second Military Medical University, Shanghai, ChinaShanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United StatesShanghai Institute of Immunology, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Rheumatology and Immunology, Shanghai Chang Zheng Hospital, Second Military Medical University, Shanghai, ChinaBeijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, ChinaPeking-Tsinghua Center for Life Sciences, Tsinghua University, Beijing, ChinaTNFα inhibitors have shaped the landscape of rheumatoid arthritis (RA) therapy with high clinical efficiency. However, their impact on T cell recall responses is not well-elucidated. We aimed to analyze the immune profiles of memory T cells in RA patients undergoing TNFα inhibitor Golimumab (GM) treatment. Frequencies of peripheral T cell subsets and cytokine expression profiles in memory T cells (TM) upon PMA/Ionomycine stimulation were determined by flow cytometry. Antigen-specific CD8 T cell immunity was analyzed through stimulating PBMCs with CMV-EBV-Flu (CEF) viral peptide pool and subsequent intracellular IFNγ staining. Both peripheral CD8 and CD4 T cells from GM treated patients had a shift pattern characterized by an enlarged effector TM and a reduced central TM cell population when compared to GM untreated group. An increase in the frequencies of TNFα+, IL-2+, and IL-17+ CD8 TM cells was observed whereas only TNFα+CD4 TM cells increased in GM treated patients. Moreover, GM treated patients contained more peripheral IFNγ-producing CD8 T cells specific to CEF viral peptides. Together, these results show a distinct T cell subset pattern and enhanced memory T cell immunity upon GM treatment, suggesting an immunoregulatory effect of TNF inhibitor Golimumab on peripheral memory T cell responses.https://www.frontiersin.org/article/10.3389/fimmu.2019.00887/fullrheumatoid arthritismemory T cellsantigen specificTNFα inhibitorimmune regulation |
| spellingShingle | Asma Khanniche Asma Khanniche Ling Zhou Bin Jiang Jing Song Yanhua Jin Jian Yin Shujun Wang Ping Ji Hao Shen Ying Wang Huji Xu Huji Xu Huji Xu Restored and Enhanced Memory T Cell Immunity in Rheumatoid Arthritis After TNFα Blocker Treatment Frontiers in Immunology rheumatoid arthritis memory T cells antigen specific TNFα inhibitor immune regulation |
| title | Restored and Enhanced Memory T Cell Immunity in Rheumatoid Arthritis After TNFα Blocker Treatment |
| title_full | Restored and Enhanced Memory T Cell Immunity in Rheumatoid Arthritis After TNFα Blocker Treatment |
| title_fullStr | Restored and Enhanced Memory T Cell Immunity in Rheumatoid Arthritis After TNFα Blocker Treatment |
| title_full_unstemmed | Restored and Enhanced Memory T Cell Immunity in Rheumatoid Arthritis After TNFα Blocker Treatment |
| title_short | Restored and Enhanced Memory T Cell Immunity in Rheumatoid Arthritis After TNFα Blocker Treatment |
| title_sort | restored and enhanced memory t cell immunity in rheumatoid arthritis after tnfα blocker treatment |
| topic | rheumatoid arthritis memory T cells antigen specific TNFα inhibitor immune regulation |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.00887/full |
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