Authentication of chicken-derived components in collagen-containing foods using natural macromolecular marker fragments by LC-MS method

Collagen is a biopolymer in animals that has been widely used in the food field to improve the value of health benefits in products. Chicken skin, which is a collagen-containing food, is popular as a healthy fat replacer for processed meat. To enable high information transparency of the products, th...

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Main Authors: Guiya Deng, Xianchao Jiang, Zihao Song, Chengming Li, Yuepeng Guan, Shangwei Guo, Yaqin Huang
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:Polymer Testing
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0142941823000302
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author Guiya Deng
Xianchao Jiang
Zihao Song
Chengming Li
Yuepeng Guan
Shangwei Guo
Yaqin Huang
author_facet Guiya Deng
Xianchao Jiang
Zihao Song
Chengming Li
Yuepeng Guan
Shangwei Guo
Yaqin Huang
author_sort Guiya Deng
collection DOAJ
description Collagen is a biopolymer in animals that has been widely used in the food field to improve the value of health benefits in products. Chicken skin, which is a collagen-containing food, is popular as a healthy fat replacer for processed meat. To enable high information transparency of the products, the chicken-derived components need to be traceable to guarantee food safety and quality. However, the current methods for identifying the chicken-derived components in food still lack accuracy. Here a combination of two marker peptides were discovered and employed to establish a sensitive method based on LC-MS for the detection of chicken-derived components in collagen-containing foods. This method has a very low detection limit of 0.33 pg and 0.16 pg for two marker peptides, respectively. The results illustrated that the combination of these maker peptides could be utilized for the authentication of the chicken skin-derived components in processed meat.
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spelling doaj.art-6e83b5161d2c42ec88e15e9fcd24c44c2023-03-13T04:15:00ZengElsevierPolymer Testing0142-94182023-03-01120107950Authentication of chicken-derived components in collagen-containing foods using natural macromolecular marker fragments by LC-MS methodGuiya Deng0Xianchao Jiang1Zihao Song2Chengming Li3Yuepeng Guan4Shangwei Guo5Yaqin Huang6Beijing Laboratory of Biomedical Materials, Key Laboratory of Biomedical Materials of Natural Macromolecules (Beijing University of Chemical Technology), Ministry of Education, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of ChinaBeijing Laboratory of Biomedical Materials, Key Laboratory of Biomedical Materials of Natural Macromolecules (Beijing University of Chemical Technology), Ministry of Education, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of ChinaBeijing Laboratory of Biomedical Materials, Key Laboratory of Biomedical Materials of Natural Macromolecules (Beijing University of Chemical Technology), Ministry of Education, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of ChinaBeijing Laboratory of Biomedical Materials, Key Laboratory of Biomedical Materials of Natural Macromolecules (Beijing University of Chemical Technology), Ministry of Education, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of ChinaBeijing Key Laboratory of Clothing Materials R&D and Assessment, Beijing Engineering Research Center of Textile Nano Fiber, Beijing Institute of Fashion Technology, Beijing, 100029, People's Republic of China; Corresponding author.National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, Dong-E-Ejiao Co.,Ltd., Liaocheng, 252201, People's Republic of China; Corresponding author.Beijing Laboratory of Biomedical Materials, Key Laboratory of Biomedical Materials of Natural Macromolecules (Beijing University of Chemical Technology), Ministry of Education, Beijing University of Chemical Technology, Beijing, 100029, People's Republic of China; Corresponding author.Collagen is a biopolymer in animals that has been widely used in the food field to improve the value of health benefits in products. Chicken skin, which is a collagen-containing food, is popular as a healthy fat replacer for processed meat. To enable high information transparency of the products, the chicken-derived components need to be traceable to guarantee food safety and quality. However, the current methods for identifying the chicken-derived components in food still lack accuracy. Here a combination of two marker peptides were discovered and employed to establish a sensitive method based on LC-MS for the detection of chicken-derived components in collagen-containing foods. This method has a very low detection limit of 0.33 pg and 0.16 pg for two marker peptides, respectively. The results illustrated that the combination of these maker peptides could be utilized for the authentication of the chicken skin-derived components in processed meat.http://www.sciencedirect.com/science/article/pii/S0142941823000302CollagenMarker peptideAuthenticationAnimal origin
spellingShingle Guiya Deng
Xianchao Jiang
Zihao Song
Chengming Li
Yuepeng Guan
Shangwei Guo
Yaqin Huang
Authentication of chicken-derived components in collagen-containing foods using natural macromolecular marker fragments by LC-MS method
Polymer Testing
Collagen
Marker peptide
Authentication
Animal origin
title Authentication of chicken-derived components in collagen-containing foods using natural macromolecular marker fragments by LC-MS method
title_full Authentication of chicken-derived components in collagen-containing foods using natural macromolecular marker fragments by LC-MS method
title_fullStr Authentication of chicken-derived components in collagen-containing foods using natural macromolecular marker fragments by LC-MS method
title_full_unstemmed Authentication of chicken-derived components in collagen-containing foods using natural macromolecular marker fragments by LC-MS method
title_short Authentication of chicken-derived components in collagen-containing foods using natural macromolecular marker fragments by LC-MS method
title_sort authentication of chicken derived components in collagen containing foods using natural macromolecular marker fragments by lc ms method
topic Collagen
Marker peptide
Authentication
Animal origin
url http://www.sciencedirect.com/science/article/pii/S0142941823000302
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