16S rRNA gene primer choice impacts off-target amplification in human gastrointestinal tract biopsies and microbiome profiling

16S rRNA gene primer choice impacts off-target amplification in human gastrointestinal tract biopsies and microbiome profiling

Abstract 16S rRNA amplicon sequencing or, more recently, metatranscriptomic analysis are currently the only preferred methods for microbial profiling of samples containing a predominant ratio of human to bacterial DNA. However, due to the off-target amplification of human DNA, current protocols are...

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Bibliographic Details
Main Authors: Tereza Deissová, Martina Zapletalová, Lumír Kunovský, Radek Kroupa, Tomáš Grolich, Zdeněk Kala, Petra Bořilová Linhartová, Jan Lochman
Format: Article
Language:English
Published: Nature Portfolio 2023-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-39575-8

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https://doi.org/10.1038/s41598-023-39575-8

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