A comprehensive assessment of RNA-seq protocols for degraded and low-quantity samples

A comprehensive assessment of RNA-seq protocols for degraded and low-quantity samples

Abstract Background RNA-sequencing (RNA-seq) has emerged as one of the most sensitive tool for gene expression analysis. Among the library preparation methods available, the standard poly(A) + enrichment provides a comprehensive, detailed, and accurate view of polyadenylated RNAs. However, on sample...

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Bibliographic Details
Main Authors: Sven Schuierer, Walter Carbone, Judith Knehr, Virginie Petitjean, Anita Fernandez, Marc Sultan, Guglielmo Roma
Format: Article
Language:English
Published: BMC 2017-06-01
Series:BMC Genomics
Subjects:
RNA-sequencing
Expression profiling
Benchmarking
Low quality
Low quantity
Differential expression
Online Access:http://link.springer.com/article/10.1186/s12864-017-3827-y

Internet

http://link.springer.com/article/10.1186/s12864-017-3827-y

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