| Summary: | Background Toxicity evaluation based on two-dimensional cell culture shows differences from clinical results and has the disadvantage of not accurately reflecting cell-to-cell cross-signaling. Since almost all cells in the human body are arranged in a three-dimensional structure and constitute a tissue, the in vitro reproduction of a three-dimensional tissue composed of human cells can be used as an effective model for drug development and toxicity evaluation. The clearing technique improves image resolution and can implement three-dimensional bio-images throughout the organization, enabling more efficient toxicity evaluation of disease model analysis using spheroids. Methods We generate an 100-200um spheroids and optimized clearing condition. Next application and imaging of optimized clearing condition in the cardiac spheroid model. Results Here we first reported that the optical spheroid clearing protocol for the image-based toxicity prediction model. In our results, spheroid clearing significantly increases fluorescence intensity and enables image-based toxicity prediction. Conclusion We propose that these clearing methods of spheroid can be utilized for the image-based cardiotoxicity evaluation. Furthermore, we also present the possibility that our protocol can also be utilized for patient-tailored toxicity prediction evaluation.
|
|---|