Evidence against a human cell-specific role for LRP6 in anthrax toxin entry.
The role of the cellular protein LRP6 in anthrax toxin entry is controversial. Previous studies showed that LRP6 was important for efficient intoxication of human M2182 prostate carcinoma cells but other studies performed with cells from gene-knockout mice demonstrated no role for either LRP6 or the...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-03-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2263128?pdf=render |
| _version_ | 1811221598850514944 |
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| author | Patricia L Ryan John A T Young |
| author_facet | Patricia L Ryan John A T Young |
| author_sort | Patricia L Ryan |
| collection | DOAJ |
| description | The role of the cellular protein LRP6 in anthrax toxin entry is controversial. Previous studies showed that LRP6 was important for efficient intoxication of human M2182 prostate carcinoma cells but other studies performed with cells from gene-knockout mice demonstrated no role for either LRP6 or the related LRP5 protein in anthrax toxin entry. One possible explanation for this discrepancy is that LRP6 may be important for anthrax toxin entry into human, but not mouse, cells. To test this idea we have investigated the effect of knocking down LRP6 or LRP5 expression with siRNAs in human HeLa cells. We show here that efficient knockdown of either LRP6, LRP5, or both proteins has no influence on the kinetics of anthrax lethal toxin entry or MEK1 substrate cleavage in these cells. These data argue against a human-specific role for LRP6 in anthrax toxin entry and suggest instead that involvement of this protein may be restricted to certain cell types independently of their species of origin. |
| first_indexed | 2024-04-12T08:02:09Z |
| format | Article |
| id | doaj.art-6eb3f1e68dde407d98a59a33ca27ede1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T08:02:09Z |
| publishDate | 2008-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6eb3f1e68dde407d98a59a33ca27ede12022-12-22T03:41:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-03-0133e181710.1371/journal.pone.0001817Evidence against a human cell-specific role for LRP6 in anthrax toxin entry.Patricia L RyanJohn A T YoungThe role of the cellular protein LRP6 in anthrax toxin entry is controversial. Previous studies showed that LRP6 was important for efficient intoxication of human M2182 prostate carcinoma cells but other studies performed with cells from gene-knockout mice demonstrated no role for either LRP6 or the related LRP5 protein in anthrax toxin entry. One possible explanation for this discrepancy is that LRP6 may be important for anthrax toxin entry into human, but not mouse, cells. To test this idea we have investigated the effect of knocking down LRP6 or LRP5 expression with siRNAs in human HeLa cells. We show here that efficient knockdown of either LRP6, LRP5, or both proteins has no influence on the kinetics of anthrax lethal toxin entry or MEK1 substrate cleavage in these cells. These data argue against a human-specific role for LRP6 in anthrax toxin entry and suggest instead that involvement of this protein may be restricted to certain cell types independently of their species of origin.http://europepmc.org/articles/PMC2263128?pdf=render |
| spellingShingle | Patricia L Ryan John A T Young Evidence against a human cell-specific role for LRP6 in anthrax toxin entry. PLoS ONE |
| title | Evidence against a human cell-specific role for LRP6 in anthrax toxin entry. |
| title_full | Evidence against a human cell-specific role for LRP6 in anthrax toxin entry. |
| title_fullStr | Evidence against a human cell-specific role for LRP6 in anthrax toxin entry. |
| title_full_unstemmed | Evidence against a human cell-specific role for LRP6 in anthrax toxin entry. |
| title_short | Evidence against a human cell-specific role for LRP6 in anthrax toxin entry. |
| title_sort | evidence against a human cell specific role for lrp6 in anthrax toxin entry |
| url | http://europepmc.org/articles/PMC2263128?pdf=render |
| work_keys_str_mv | AT patricialryan evidenceagainstahumancellspecificroleforlrp6inanthraxtoxinentry AT johnatyoung evidenceagainstahumancellspecificroleforlrp6inanthraxtoxinentry |