基于组合优化策略在毕赤酵母中高效表达 杜邦嗜热菌脂肪酶High-efficient expression of Thermomyces dupontii lipase in Pichia pastoris based on combinatorial optimization strategy

基于组合优化策略在毕赤酵母中高效表达 杜邦嗜热菌脂肪酶High-efficient expression of Thermomyces dupontii lipase in Pichia pastoris based on combinatorial optimization strategy

杜邦嗜热菌脂肪酶(LIP1)是一种在洗涤行业、生物柴油制备、油脂改性等领域具有良好应用潜力的碱性脂肪酶，然而其天然产量极低，难以满足产业化需求。采用组合优化策略筛选出高效表达LIP1的毕赤酵母重组菌株。首先，构建毕赤酵母密码子偏好性优化的LIP1基因，通过高浓度G418抗性平板和BMMY-罗丹明B定性平板筛选出脂肪酶活性较高的重组菌株；其次，利用信号肽优化和分子伴侣共表达优化筛选得到一株高效表达的重组毕赤酵母菌株GS115/pPIC9K-Mss-SSA4-LIP1，采用碱滴定法测定其酶活,采用比色法测定其底物特异性。结果表明，经组合优化策略筛选出的菌株在摇瓶和5 L发酵罐中发酵最高分泌酶活分别...

Full description

Bibliographic Details
Main Author: 汪步青1，陈洲1，王亚森1，许向阳2，高晓冬1，藤田盛久1，李子杰1WANG Buqing1, CHEN Zhou1, WANG Yasen1, XU Xiangyang2, GAO Xiaodong1, FUJITA Morihisa1, LI Zijie1
Format: Article
Language:English
Published: 中粮工科（西安）国际工程有限公司 2022-07-01
Series:Zhongguo youzhi
Subjects:
杜邦嗜热菌脂肪酶（lip1）；组合优化策略；毕赤酵母；高密度表达；底物特异性
thermomyces dupontii lipase (lip1); combinatorial optimization strategy; pichia pastoris; high-density expression; substrate specificity
Online Access:http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20220724&year_id=2022&quarter_id=7&falg=1

Internet

http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20220724&year_id=2022&quarter_id=7&falg=1

Similar Items