Alt a 1 Promotes Allergic Asthma In Vivo Through TLR4-Alveolar Macrophages
The mold Alternaria alternata is one of the main sources of asthma exacerbation, being its major allergen, Alt a 1, indispensable for its development. The main objective of this work was to answer two main questions: 1) can Alt a 1 by itself (without any other context) induce an asthmatic profile in...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.877383/full |
| _version_ | 1818242389213446144 |
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| author | Guadalupe Hernandez-Ramirez Diego Pazos-Castro Diego Pazos-Castro Zulema Gonzalez-Klein Zulema Gonzalez-Klein Jose Luis Resuela-Gonzalez Sergio Fernandez-Bravo Lucia Palacio-Garcia Vanesa Esteban Maria Garrido-Arandia Maria Garrido-Arandia Jaime Tome-Amat Araceli Diaz-Perales Araceli Diaz-Perales |
| author_facet | Guadalupe Hernandez-Ramirez Diego Pazos-Castro Diego Pazos-Castro Zulema Gonzalez-Klein Zulema Gonzalez-Klein Jose Luis Resuela-Gonzalez Sergio Fernandez-Bravo Lucia Palacio-Garcia Vanesa Esteban Maria Garrido-Arandia Maria Garrido-Arandia Jaime Tome-Amat Araceli Diaz-Perales Araceli Diaz-Perales |
| author_sort | Guadalupe Hernandez-Ramirez |
| collection | DOAJ |
| description | The mold Alternaria alternata is one of the main sources of asthma exacerbation, being its major allergen, Alt a 1, indispensable for its development. The main objective of this work was to answer two main questions: 1) can Alt a 1 by itself (without any other context) induce an asthmatic profile in vivo?; and 2) Which molecular mechanisms take place during this phenomenon? To answer both questions, we have developed a mouse model of allergic asthma using only Alt a 1 for mice sensitization. We also made use of in-vitro cellular models and computational studies to support some aspects of our hypothesis. Our results showed that Alt a 1 can induce an asthmatic phenotype, promoting tissue remodeling and infiltration of CD45+ cells, especially eosinophils and macrophages (Siglec F+ and F4/80+). Also, we have found that Alt a 1 sensitization is mediated by the TLR4-macrophage axis. |
| first_indexed | 2024-12-12T13:44:27Z |
| format | Article |
| id | doaj.art-6ec1b4f30ba447a3956e36249081be15 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-12T13:44:27Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-6ec1b4f30ba447a3956e36249081be152022-12-22T00:22:43ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-06-011310.3389/fimmu.2022.877383877383Alt a 1 Promotes Allergic Asthma In Vivo Through TLR4-Alveolar MacrophagesGuadalupe Hernandez-Ramirez0Diego Pazos-Castro1Diego Pazos-Castro2Zulema Gonzalez-Klein3Zulema Gonzalez-Klein4Jose Luis Resuela-Gonzalez5Sergio Fernandez-Bravo6Lucia Palacio-Garcia7Vanesa Esteban8Maria Garrido-Arandia9Maria Garrido-Arandia10Jaime Tome-Amat11Araceli Diaz-Perales12Araceli Diaz-Perales13Centre for Plant Biotechnology and Genomics Universidad Politécnica de Madrid, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Consejo Superior de Investigaciones Cientificas (UPM –INIA/CSIC), Universidad Politécnica de Madrid, Madrid, SpainCentre for Plant Biotechnology and Genomics Universidad Politécnica de Madrid, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Consejo Superior de Investigaciones Cientificas (UPM –INIA/CSIC), Universidad Politécnica de Madrid, Madrid, SpainDepartment of Biotechnology-Plant Biology, Escuela Tecnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas (ETSIAAB), Universidad Politécnica de Madrid, Madrid, SpainCentre for Plant Biotechnology and Genomics Universidad Politécnica de Madrid, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Consejo Superior de Investigaciones Cientificas (UPM –INIA/CSIC), Universidad Politécnica de Madrid, Madrid, SpainDepartment of Biotechnology-Plant Biology, Escuela Tecnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas (ETSIAAB), Universidad Politécnica de Madrid, Madrid, SpainCentre for Plant Biotechnology and Genomics Universidad Politécnica de Madrid, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Consejo Superior de Investigaciones Cientificas (UPM –INIA/CSIC), Universidad Politécnica de Madrid, Madrid, SpainDepartment of Allergy and Immunology, IIS-Fundación Jiménez Díaz, Universidad Autónoma de Madrid (UAM), Madrid, SpainDepartment of Allergy and Immunology, IIS-Fundación Jiménez Díaz, Universidad Autónoma de Madrid (UAM), Madrid, SpainDepartment of Allergy and Immunology, IIS-Fundación Jiménez Díaz, Universidad Autónoma de Madrid (UAM), Madrid, SpainCentre for Plant Biotechnology and Genomics Universidad Politécnica de Madrid, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Consejo Superior de Investigaciones Cientificas (UPM –INIA/CSIC), Universidad Politécnica de Madrid, Madrid, SpainDepartment of Biotechnology-Plant Biology, Escuela Tecnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas (ETSIAAB), Universidad Politécnica de Madrid, Madrid, SpainCentre for Plant Biotechnology and Genomics Universidad Politécnica de Madrid, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Consejo Superior de Investigaciones Cientificas (UPM –INIA/CSIC), Universidad Politécnica de Madrid, Madrid, SpainCentre for Plant Biotechnology and Genomics Universidad Politécnica de Madrid, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Consejo Superior de Investigaciones Cientificas (UPM –INIA/CSIC), Universidad Politécnica de Madrid, Madrid, SpainDepartment of Biotechnology-Plant Biology, Escuela Tecnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas (ETSIAAB), Universidad Politécnica de Madrid, Madrid, SpainThe mold Alternaria alternata is one of the main sources of asthma exacerbation, being its major allergen, Alt a 1, indispensable for its development. The main objective of this work was to answer two main questions: 1) can Alt a 1 by itself (without any other context) induce an asthmatic profile in vivo?; and 2) Which molecular mechanisms take place during this phenomenon? To answer both questions, we have developed a mouse model of allergic asthma using only Alt a 1 for mice sensitization. We also made use of in-vitro cellular models and computational studies to support some aspects of our hypothesis. Our results showed that Alt a 1 can induce an asthmatic phenotype, promoting tissue remodeling and infiltration of CD45+ cells, especially eosinophils and macrophages (Siglec F+ and F4/80+). Also, we have found that Alt a 1 sensitization is mediated by the TLR4-macrophage axis.https://www.frontiersin.org/articles/10.3389/fimmu.2022.877383/fullallergic asthmaAlt a 1TLR4alveolar macrophagemouse model |
| spellingShingle | Guadalupe Hernandez-Ramirez Diego Pazos-Castro Diego Pazos-Castro Zulema Gonzalez-Klein Zulema Gonzalez-Klein Jose Luis Resuela-Gonzalez Sergio Fernandez-Bravo Lucia Palacio-Garcia Vanesa Esteban Maria Garrido-Arandia Maria Garrido-Arandia Jaime Tome-Amat Araceli Diaz-Perales Araceli Diaz-Perales Alt a 1 Promotes Allergic Asthma In Vivo Through TLR4-Alveolar Macrophages Frontiers in Immunology allergic asthma Alt a 1 TLR4 alveolar macrophage mouse model |
| title | Alt a 1 Promotes Allergic Asthma In Vivo Through TLR4-Alveolar Macrophages |
| title_full | Alt a 1 Promotes Allergic Asthma In Vivo Through TLR4-Alveolar Macrophages |
| title_fullStr | Alt a 1 Promotes Allergic Asthma In Vivo Through TLR4-Alveolar Macrophages |
| title_full_unstemmed | Alt a 1 Promotes Allergic Asthma In Vivo Through TLR4-Alveolar Macrophages |
| title_short | Alt a 1 Promotes Allergic Asthma In Vivo Through TLR4-Alveolar Macrophages |
| title_sort | alt a 1 promotes allergic asthma in vivo through tlr4 alveolar macrophages |
| topic | allergic asthma Alt a 1 TLR4 alveolar macrophage mouse model |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.877383/full |
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