Gold In-and-Out: A Toolkit for Analyzing Subcellular Distribution of Immunogold-Labeled Membrane Proteins in Freeze-Fracture Replica Images

Gold In-and-Out: A Toolkit for Analyzing Subcellular Distribution of Immunogold-Labeled Membrane Proteins in Freeze-Fracture Replica Images

Integral membrane proteins such as ion channels, transporters, and receptors shape cell activity and mediate cell-to-cell communication in the brain. The distribution, quantity, and clustering arrangement of those proteins contribute to the physiological properties of the cell; therefore, precise qu...

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Bibliographic Details
Main Authors: Debbie Guerrero-Given, Seth L. Goldin, Connon I. Thomas, Skylar A. Anthony, Diego Jerez, Naomi Kamasawa
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-04-01
Series:Frontiers in Neuroanatomy
Subjects:
immunogold particles
image analysis
toolkit
freeze-fracture replica immunogold labeling
GUI (graphical user interface)
Online Access:https://www.frontiersin.org/articles/10.3389/fnana.2022.855218/full

Internet

https://www.frontiersin.org/articles/10.3389/fnana.2022.855218/full

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