Creation of targeted inversion mutations in plants using an RNA-guided endonuclease
Inversions are DNA rearrangements that are essential for plant gene evolution and adaptation to environmental changes. We demonstrate the creation of targeted inversions and previously reported targeted deletion mutations via delivery of a pair of RNA-guided endonucleases (RGENs) of CRISPR/Cas9. The...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2017-02-01
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| Series: | Crop Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514116301064 |
| _version_ | 1818418447642525696 |
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| author | Congsheng Zhang Changlin Liu Jianfeng Weng Beijiu Cheng Fang Liu Xinhai Li Chuanxiao Xie |
| author_facet | Congsheng Zhang Changlin Liu Jianfeng Weng Beijiu Cheng Fang Liu Xinhai Li Chuanxiao Xie |
| author_sort | Congsheng Zhang |
| collection | DOAJ |
| description | Inversions are DNA rearrangements that are essential for plant gene evolution and adaptation to environmental changes. We demonstrate the creation of targeted inversions and previously reported targeted deletion mutations via delivery of a pair of RNA-guided endonucleases (RGENs) of CRISPR/Cas9. The efficiencies of the targeted inversions were 2.6% and 2.2% in the Arabidopsis FLOWERING TIME (AtFT) and TERMINAL FLOWER 1 (AtTFL1) loci, respectively. Thus, we successfully established an approach that can potentially be used to introduce targeted DNA inversions of interest for functional studies and crop improvement. |
| first_indexed | 2024-12-14T12:22:49Z |
| format | Article |
| id | doaj.art-6ee21532e3ea443c851a36b0cdf7d5f5 |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-12-14T12:22:49Z |
| publishDate | 2017-02-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-6ee21532e3ea443c851a36b0cdf7d5f52022-12-21T23:01:25ZengKeAi Communications Co., Ltd.Crop Journal2214-51412017-02-0151838810.1016/j.cj.2016.08.001Creation of targeted inversion mutations in plants using an RNA-guided endonucleaseCongsheng Zhang0Changlin Liu1Jianfeng Weng2Beijiu Cheng3Fang Liu4Xinhai Li5Chuanxiao Xie6Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, ChinaAnhui Agricultural University, Hefei 230036, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, ChinaInversions are DNA rearrangements that are essential for plant gene evolution and adaptation to environmental changes. We demonstrate the creation of targeted inversions and previously reported targeted deletion mutations via delivery of a pair of RNA-guided endonucleases (RGENs) of CRISPR/Cas9. The efficiencies of the targeted inversions were 2.6% and 2.2% in the Arabidopsis FLOWERING TIME (AtFT) and TERMINAL FLOWER 1 (AtTFL1) loci, respectively. Thus, we successfully established an approach that can potentially be used to introduce targeted DNA inversions of interest for functional studies and crop improvement.http://www.sciencedirect.com/science/article/pii/S2214514116301064RNA-guided endonucleaseCRISPR/Cas9Targeted inversionGenetic improvement |
| spellingShingle | Congsheng Zhang Changlin Liu Jianfeng Weng Beijiu Cheng Fang Liu Xinhai Li Chuanxiao Xie Creation of targeted inversion mutations in plants using an RNA-guided endonuclease Crop Journal RNA-guided endonuclease CRISPR/Cas9 Targeted inversion Genetic improvement |
| title | Creation of targeted inversion mutations in plants using an RNA-guided endonuclease |
| title_full | Creation of targeted inversion mutations in plants using an RNA-guided endonuclease |
| title_fullStr | Creation of targeted inversion mutations in plants using an RNA-guided endonuclease |
| title_full_unstemmed | Creation of targeted inversion mutations in plants using an RNA-guided endonuclease |
| title_short | Creation of targeted inversion mutations in plants using an RNA-guided endonuclease |
| title_sort | creation of targeted inversion mutations in plants using an rna guided endonuclease |
| topic | RNA-guided endonuclease CRISPR/Cas9 Targeted inversion Genetic improvement |
| url | http://www.sciencedirect.com/science/article/pii/S2214514116301064 |
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