Simple and Efficient Targeting of Multiple Genes Through CRISPR-Cas9 in Physcomitrella patens

Powerful genome editing technologies are needed for efficient gene function analysis. The CRISPR-Cas9 system has been adapted as an efficient gene-knock-out technology in a variety of species. However, in a number of situations, knocking out or modifying a single gene is not sufficient; this is part...

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Bibliographic Details
Main Authors: Mauricio Lopez-Obando, Beate Hoffmann, Carine Géry, Anouchka Guyon-Debast, Evelyne Téoulé, Catherine Rameau, Sandrine Bonhomme, Fabien Nogué
Format: Article
Language:English
Published: Oxford University Press 2016-11-01
Series:G3: Genes, Genomes, Genetics
Subjects:
Online Access:http://g3journal.org/lookup/doi/10.1534/g3.116.033266