The enrichment of the gut microbiota Lachnoclostridium is associated with the presence of intratumoral tertiary lymphoid structures in hepatocellular carcinoma
Backgrounds and aimsImmunotherapies have formed an entirely new treatment paradigm for hepatocellular carcinoma (HCC). Tertiary lymphoid structure (TLS) has been associated with good response to immunotherapy in most solid tumors. Nonetheless, the role of TLS in human HCC remains controversial, and...
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1289753/full |
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| author | Rui Zhao Jiacheng Li Bo Chen Jungang Zhao Leyin Hu Kate Huang Qiwen Chen Jiangqiao Yao Ganglian Lin Lishimeng Bao Mengmeng Lu Yi Wang Yi Wang Gang Chen Gang Chen Gang Chen Fang Wu |
| author_facet | Rui Zhao Jiacheng Li Bo Chen Jungang Zhao Leyin Hu Kate Huang Qiwen Chen Jiangqiao Yao Ganglian Lin Lishimeng Bao Mengmeng Lu Yi Wang Yi Wang Gang Chen Gang Chen Gang Chen Fang Wu |
| author_sort | Rui Zhao |
| collection | DOAJ |
| description | Backgrounds and aimsImmunotherapies have formed an entirely new treatment paradigm for hepatocellular carcinoma (HCC). Tertiary lymphoid structure (TLS) has been associated with good response to immunotherapy in most solid tumors. Nonetheless, the role of TLS in human HCC remains controversial, and recent studies suggest that their functional heterogeneity may relate to different locations within the tumor. Exploring factors that influence the formation of TLS in HCC may provide more useful insights. However, factors affecting the presence of TLSs are still unclear. The human gut microbiota can regulate the host immune system and is associated with the efficacy of immunotherapy but, in HCC, whether the gut microbiota is related to the presence of TLS still lacks sufficient evidence.MethodsWe performed pathological examinations of tumor and para-tumor tissue sections. Based on the location of TLS in tissues, all patients were divided into intratumoral TLS (It-TLS) group and desertic TLS (De-TLS) group. According to the grouping results, we statistically analyzed the clinical, biological, and pathological features; preoperative gut microbiota data; and postoperative pathological features of patients.ResultsIn a retrospective study cohort of 60 cases from a single center, differential microbiota analysis showed that compared with the De-TLS group, the abundance of Lachnoclostridium, Hungatella, Blautia, Fusobacterium, and Clostridium was increased in the It-TLS group. Among them, the enrichment of Lachnoclostridium was the most significant and was unrelated to the clinical, biological, and pathological features of the patients. It can be seen that the difference in abundance levels of microbiota is related to the presence of TLS.ConclusionOur findings prove the enrichment of Lachnoclostridium-dominated gut microbiota is associated with the presence of It-TLS in HCC patients. |
| first_indexed | 2024-03-09T02:56:05Z |
| format | Article |
| id | doaj.art-6efe1d583708410398160feafdc6a64e |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-09T02:56:05Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-6efe1d583708410398160feafdc6a64e2023-12-05T04:26:08ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-12-011410.3389/fimmu.2023.12897531289753The enrichment of the gut microbiota Lachnoclostridium is associated with the presence of intratumoral tertiary lymphoid structures in hepatocellular carcinomaRui Zhao0Jiacheng Li1Bo Chen2Jungang Zhao3Leyin Hu4Kate Huang5Qiwen Chen6Jiangqiao Yao7Ganglian Lin8Lishimeng Bao9Mengmeng Lu10Yi Wang11Yi Wang12Gang Chen13Gang Chen14Gang Chen15Fang Wu16Department of Gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Pathology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Pathology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaThe Second Clinical College, Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Epidemiology and Biostatistics, School of Public Health and Management, Wenzhou Medical University, Wenzhou, ChinaZhejiang-Germany Interdisciplinary Joint Laboratory of Hepatobiliary-Pancreatic Tumor and Bioengineering, Wenzhou, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaZhejiang-Germany Interdisciplinary Joint Laboratory of Hepatobiliary-Pancreatic Tumor and Bioengineering, Wenzhou, ChinaKey Laboratory of Diagnosis and Treatment of Severe Hepato-Pancreatic Diseases of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaBackgrounds and aimsImmunotherapies have formed an entirely new treatment paradigm for hepatocellular carcinoma (HCC). Tertiary lymphoid structure (TLS) has been associated with good response to immunotherapy in most solid tumors. Nonetheless, the role of TLS in human HCC remains controversial, and recent studies suggest that their functional heterogeneity may relate to different locations within the tumor. Exploring factors that influence the formation of TLS in HCC may provide more useful insights. However, factors affecting the presence of TLSs are still unclear. The human gut microbiota can regulate the host immune system and is associated with the efficacy of immunotherapy but, in HCC, whether the gut microbiota is related to the presence of TLS still lacks sufficient evidence.MethodsWe performed pathological examinations of tumor and para-tumor tissue sections. Based on the location of TLS in tissues, all patients were divided into intratumoral TLS (It-TLS) group and desertic TLS (De-TLS) group. According to the grouping results, we statistically analyzed the clinical, biological, and pathological features; preoperative gut microbiota data; and postoperative pathological features of patients.ResultsIn a retrospective study cohort of 60 cases from a single center, differential microbiota analysis showed that compared with the De-TLS group, the abundance of Lachnoclostridium, Hungatella, Blautia, Fusobacterium, and Clostridium was increased in the It-TLS group. Among them, the enrichment of Lachnoclostridium was the most significant and was unrelated to the clinical, biological, and pathological features of the patients. It can be seen that the difference in abundance levels of microbiota is related to the presence of TLS.ConclusionOur findings prove the enrichment of Lachnoclostridium-dominated gut microbiota is associated with the presence of It-TLS in HCC patients.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1289753/fullhepatocellular carcinoma (HCC)tumor immune microenvironment (TIME)tertiary lymphoid structure (TLS)gut microbiotaLachnoclostridium |
| spellingShingle | Rui Zhao Jiacheng Li Bo Chen Jungang Zhao Leyin Hu Kate Huang Qiwen Chen Jiangqiao Yao Ganglian Lin Lishimeng Bao Mengmeng Lu Yi Wang Yi Wang Gang Chen Gang Chen Gang Chen Fang Wu The enrichment of the gut microbiota Lachnoclostridium is associated with the presence of intratumoral tertiary lymphoid structures in hepatocellular carcinoma Frontiers in Immunology hepatocellular carcinoma (HCC) tumor immune microenvironment (TIME) tertiary lymphoid structure (TLS) gut microbiota Lachnoclostridium |
| title | The enrichment of the gut microbiota Lachnoclostridium is associated with the presence of intratumoral tertiary lymphoid structures in hepatocellular carcinoma |
| title_full | The enrichment of the gut microbiota Lachnoclostridium is associated with the presence of intratumoral tertiary lymphoid structures in hepatocellular carcinoma |
| title_fullStr | The enrichment of the gut microbiota Lachnoclostridium is associated with the presence of intratumoral tertiary lymphoid structures in hepatocellular carcinoma |
| title_full_unstemmed | The enrichment of the gut microbiota Lachnoclostridium is associated with the presence of intratumoral tertiary lymphoid structures in hepatocellular carcinoma |
| title_short | The enrichment of the gut microbiota Lachnoclostridium is associated with the presence of intratumoral tertiary lymphoid structures in hepatocellular carcinoma |
| title_sort | enrichment of the gut microbiota lachnoclostridium is associated with the presence of intratumoral tertiary lymphoid structures in hepatocellular carcinoma |
| topic | hepatocellular carcinoma (HCC) tumor immune microenvironment (TIME) tertiary lymphoid structure (TLS) gut microbiota Lachnoclostridium |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1289753/full |
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