Spliceosomal GTPase Eftud2 regulates microglial activation and polarization
Elongation factor Tu GTP binding domain protein 2 (Eftud2) is a spliceosomal GTPase that serves as an innate immune modulator restricting virus infection. Microglia are the resident innate immune cells and the key players of immune response in the central nervous system. However, the role of Eftud2...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2023;volume=18;issue=4;spage=856;epage=862;aulast=Yang |
| _version_ | 1798021638797656064 |
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| author | Guo-Chao Yang Yuan Shi Chao-Nan Fan Ying Li Meng-Qi Yuan Jie Pei Yan Wu Hai-Tao Wu |
| author_facet | Guo-Chao Yang Yuan Shi Chao-Nan Fan Ying Li Meng-Qi Yuan Jie Pei Yan Wu Hai-Tao Wu |
| author_sort | Guo-Chao Yang |
| collection | DOAJ |
| description | Elongation factor Tu GTP binding domain protein 2 (Eftud2) is a spliceosomal GTPase that serves as an innate immune modulator restricting virus infection. Microglia are the resident innate immune cells and the key players of immune response in the central nervous system. However, the role of Eftud2 in microglia has not been reported. In this study, we performed immunofluorescent staining and western blot assay and found that Eftud2 was upregulated in microglia of a 5xFAD transgenic mouse model of Alzheimer’s disease. Next, we generated an inducible microglia-specific Eftud2 conditional knockout mouse line (CX3CR1-CreER; Eftud2f/f cKO) via Cre/loxP recombination and found that Eftud2 deficiency resulted in abnormal proliferation and promoted anti-inflammatory phenotype activation of microglia. Furthermore, we knocked down Eftud2 in BV2 microglia with siRNA specifically targeting Eftud2 and found that Eftud2-mediated regulation of microglial proinflammatory/anti-inflammatory phenotype activation in response to inflammation might be dependent on the NF-κB signaling pathway. Our findings suggest that Eftud2 plays a key role in regulating microglial polarization and homeostasis possibly through the NF-κB signaling pathway. |
| first_indexed | 2024-04-11T17:16:56Z |
| format | Article |
| id | doaj.art-6f0e2978f7324600a9e53fd53101b04b |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-04-11T17:16:56Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-6f0e2978f7324600a9e53fd53101b04b2022-12-22T04:12:36ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742023-01-0118485686210.4103/1673-5374.347739Spliceosomal GTPase Eftud2 regulates microglial activation and polarizationGuo-Chao YangYuan ShiChao-Nan FanYing LiMeng-Qi YuanJie PeiYan WuHai-Tao WuElongation factor Tu GTP binding domain protein 2 (Eftud2) is a spliceosomal GTPase that serves as an innate immune modulator restricting virus infection. Microglia are the resident innate immune cells and the key players of immune response in the central nervous system. However, the role of Eftud2 in microglia has not been reported. In this study, we performed immunofluorescent staining and western blot assay and found that Eftud2 was upregulated in microglia of a 5xFAD transgenic mouse model of Alzheimer’s disease. Next, we generated an inducible microglia-specific Eftud2 conditional knockout mouse line (CX3CR1-CreER; Eftud2f/f cKO) via Cre/loxP recombination and found that Eftud2 deficiency resulted in abnormal proliferation and promoted anti-inflammatory phenotype activation of microglia. Furthermore, we knocked down Eftud2 in BV2 microglia with siRNA specifically targeting Eftud2 and found that Eftud2-mediated regulation of microglial proinflammatory/anti-inflammatory phenotype activation in response to inflammation might be dependent on the NF-κB signaling pathway. Our findings suggest that Eftud2 plays a key role in regulating microglial polarization and homeostasis possibly through the NF-κB signaling pathway.http://www.nrronline.org/article.asp?issn=1673-5374;year=2023;volume=18;issue=4;spage=856;epage=862;aulast=Yangalzheimer’s disease; anti-inflammatory phenotype; bv2; eftud2; inflammation; lipopolysaccharide; microglia; nuclear factor-kappab; proinflammatory phenotype; spliceosomal gtpase |
| spellingShingle | Guo-Chao Yang Yuan Shi Chao-Nan Fan Ying Li Meng-Qi Yuan Jie Pei Yan Wu Hai-Tao Wu Spliceosomal GTPase Eftud2 regulates microglial activation and polarization Neural Regeneration Research alzheimer’s disease; anti-inflammatory phenotype; bv2; eftud2; inflammation; lipopolysaccharide; microglia; nuclear factor-kappab; proinflammatory phenotype; spliceosomal gtpase |
| title | Spliceosomal GTPase Eftud2 regulates microglial activation and polarization |
| title_full | Spliceosomal GTPase Eftud2 regulates microglial activation and polarization |
| title_fullStr | Spliceosomal GTPase Eftud2 regulates microglial activation and polarization |
| title_full_unstemmed | Spliceosomal GTPase Eftud2 regulates microglial activation and polarization |
| title_short | Spliceosomal GTPase Eftud2 regulates microglial activation and polarization |
| title_sort | spliceosomal gtpase eftud2 regulates microglial activation and polarization |
| topic | alzheimer’s disease; anti-inflammatory phenotype; bv2; eftud2; inflammation; lipopolysaccharide; microglia; nuclear factor-kappab; proinflammatory phenotype; spliceosomal gtpase |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2023;volume=18;issue=4;spage=856;epage=862;aulast=Yang |
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