Assessment of protein inclusions in cultured cells using automated image analysis

Summary: Proteinaceous inclusions are associated with neurodegenerative diseases and cell models are often used to determine genetic and chemical modifiers of their formation. This protocol involves the usage of automated microscopy and machine learning-based image analysis to accurately quantify th...

Full description

Bibliographic Details
Main Authors: Luke McAlary, Victoria K. Shephard, Mine Sher, Lauren J. Rice, Justin J. Yerbury, Neil R. Cashman, Steven S. Plotkin
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722006281