Rational Engineering of 3α-Hydroxysteroid Dehydrogenase/Carbonyl Reductase for a Biomimetic Nicotinamide Mononucleotide Cofactor

Rational Engineering of 3α-Hydroxysteroid Dehydrogenase/Carbonyl Reductase for a Biomimetic Nicotinamide Mononucleotide Cofactor

Enzymes are powerful biological catalysts for natural substrates but they have low catalytic efficiency for non-natural substrates. Protein engineering can be used to optimize enzymes for catalysis and stability. 3α-Hydroxysteroid dehydrogenase/carbonyl reductase (3α-HSD/CR) catalyzes the oxidoreduc...

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Bibliographic Details
Main Authors: Yan-Liang Chen, Yun-Hao Chou, Chia-Lin Hsieh, Shean-Jaw Chiou, Tzu-Pin Wang, Chi-Ching Hwang
Format: Article
Language:English
Published: MDPI AG 2022-09-01
Series:Catalysts
Subjects:
protein engineering
enzyme catalysis
steady-state kinetics
rational design
biomimetic cofactor
protein thermal stability
Online Access:https://www.mdpi.com/2073-4344/12/10/1094

Internet

https://www.mdpi.com/2073-4344/12/10/1094

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