Optimization of enzyme-linked immunosorbent assay kit protocol to detect trimethylamine N-oxide levels in humans

The serum level of trimethylamine N-oxide (TMAO), a gut microbiota metabolite associated with diabetes, cancer, inflammatory and neurological diseases, can be determined by the micro-enzyme-linked immunosorbent assay (ELISA) method. However, we had problems obtaining accurate standard curves with th...

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Bibliographic Details
Main Authors: Zinnet Sevval Aksoyalp, Betül Rabia Erdoğan, Saliha Aksun
Format: Article
Language:English
Published: IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund 2023-02-01
Series:EXCLI Journal : Experimental and Clinical Sciences
Subjects:
Online Access:https://www.excli.de/index.php/excli/article/view/5617