NaOH low‐salt method for chloroplast isolation and highly pure cpDNA preparation from Aeluropus littoralis

NaOH low‐salt method for chloroplast isolation and highly pure cpDNA preparation from Aeluropus littoralis

Premise of the Study High‐yield pure chloroplast DNA (cpDNA) is necessary for whole genome sequencing. Chloroplast extraction with traditional high‐salt methods causes damage to nuclei and destroys the integrity of organelles, which leads to high genomic contamination from the nucleus and mitochondr...

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Bibliographic Details
Main Authors: Najmeh Nasiri, Ghorbanali Nematzadeh, Vahid Shariati‐Joni, Hamid Najafi‐Zarrini
Format: Article
Language:English
Published: Wiley 2018-09-01
Series:Applications in Plant Sciences
Subjects:
chloroplast extraction
cpDNA preparation
high‐salt methods
Poaceae
whole genome sequencing
Online Access:https://doi.org/10.1002/aps3.1183

Internet

https://doi.org/10.1002/aps3.1183

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