Microscope 3D Point Spread Function Evaluation Method on a Confirmed Object Plane Perpendicular to the Optical Axis
A point spread function evaluation method for a microscope on the object plane that is perpendicular to the optical axis is proposed. The measurement of the incident beam direction from the dual position-sensitive-detector (PSD)-based units, the determination of the object plane perpendicularity and...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/7/2430 |
| _version_ | 1797571595117527040 |
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| author | Shuai Mao Zhenzhou Wang Jinfeng Pan |
| author_facet | Shuai Mao Zhenzhou Wang Jinfeng Pan |
| author_sort | Shuai Mao |
| collection | DOAJ |
| description | A point spread function evaluation method for a microscope on the object plane that is perpendicular to the optical axis is proposed. The measurement of the incident beam direction from the dual position-sensitive-detector (PSD)-based units, the determination of the object plane perpendicularity and the paraxial region, and evaluation methods for the point spread function (PSF) are presented and integrated into the proposed method. The experimental verification demonstrates that the proposed method can achieve a 3D PSF on the perpendicular object plane, as well as magnification, paraxial region evaluation, and confirmation for any microscopic system. |
| first_indexed | 2024-03-10T20:42:56Z |
| format | Article |
| id | doaj.art-6f28c791ddef4d748b0516c4d6767f7f |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T20:42:56Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-6f28c791ddef4d748b0516c4d6767f7f2023-11-19T20:30:21ZengMDPI AGApplied Sciences2076-34172020-04-01107243010.3390/app10072430Microscope 3D Point Spread Function Evaluation Method on a Confirmed Object Plane Perpendicular to the Optical AxisShuai Mao0Zhenzhou Wang1Jinfeng Pan2School of Electrical and Electronic Engineering, Shandong University of Technology, Zibo 255000, ChinaSchool of Electrical and Electronic Engineering, Shandong University of Technology, Zibo 255000, ChinaSchool of Electrical and Electronic Engineering, Shandong University of Technology, Zibo 255000, ChinaA point spread function evaluation method for a microscope on the object plane that is perpendicular to the optical axis is proposed. The measurement of the incident beam direction from the dual position-sensitive-detector (PSD)-based units, the determination of the object plane perpendicularity and the paraxial region, and evaluation methods for the point spread function (PSF) are presented and integrated into the proposed method. The experimental verification demonstrates that the proposed method can achieve a 3D PSF on the perpendicular object plane, as well as magnification, paraxial region evaluation, and confirmation for any microscopic system.https://www.mdpi.com/2076-3417/10/7/2430point spread functionmicroscopeoptical axisobject plane |
| spellingShingle | Shuai Mao Zhenzhou Wang Jinfeng Pan Microscope 3D Point Spread Function Evaluation Method on a Confirmed Object Plane Perpendicular to the Optical Axis Applied Sciences point spread function microscope optical axis object plane |
| title | Microscope 3D Point Spread Function Evaluation Method on a Confirmed Object Plane Perpendicular to the Optical Axis |
| title_full | Microscope 3D Point Spread Function Evaluation Method on a Confirmed Object Plane Perpendicular to the Optical Axis |
| title_fullStr | Microscope 3D Point Spread Function Evaluation Method on a Confirmed Object Plane Perpendicular to the Optical Axis |
| title_full_unstemmed | Microscope 3D Point Spread Function Evaluation Method on a Confirmed Object Plane Perpendicular to the Optical Axis |
| title_short | Microscope 3D Point Spread Function Evaluation Method on a Confirmed Object Plane Perpendicular to the Optical Axis |
| title_sort | microscope 3d point spread function evaluation method on a confirmed object plane perpendicular to the optical axis |
| topic | point spread function microscope optical axis object plane |
| url | https://www.mdpi.com/2076-3417/10/7/2430 |
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