High-Throughput Metabolic Soft-Spot Identification in Liver Microsomes by LC/UV/MS: Application of a Single Variable Incubation Time Approach

High-Throughput Metabolic Soft-Spot Identification in Liver Microsomes by LC/UV/MS: Application of a Single Variable Incubation Time Approach

CYP-mediated fast metabolism may lead to poor bioavailability, fast drug clearance and significant drug interaction. Thus, metabolic stability screening in human liver microsomes (HLM) followed by metabolic soft-spot identification (MSSID) is routinely conducted in drug discovery. Liver microsomal i...

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Bibliographic Details
Main Authors: Yanlin Zhu, Guiying Chen, Kerong Zhang, Chang Chen, Weiqing Chen, Mingshe Zhu, Hongliang Jiang
Format: Article
Language:English
Published: MDPI AG 2022-11-01
Series:Molecules
Subjects:
metabolic soft-spot
LC/UV/MS
metabolite identification
Qtrap mass spectrometry
triple-TOF mass spectrometry
Online Access:https://www.mdpi.com/1420-3049/27/22/8058

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https://www.mdpi.com/1420-3049/27/22/8058

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