Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes

Abstract Existing methods for paired antibody heavy- and light-chain repertoire sequencing rely on specialized equipment and are limited by their commercial availability and high costs. Here, we report a novel simple and cost-effective emulsion-based single-cell paired antibody repertoire sequencing...

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Bibliographic Details
Main Authors: Praneeth Reddy Devulapally, Jörg Bürger, Thorsten Mielke, Zoltán Konthur, Hans Lehrach, Marie-Laure Yaspo, Jörn Glökler, Hans-Jörg Warnatz
Format: Article
Language:English
Published: BMC 2018-04-01
Series:Genome Medicine
Online Access:http://link.springer.com/article/10.1186/s13073-018-0542-5