Impact of simulated brain interstitial fluid flow on the chemokine CXCL12 release from an alginate-based hydrogel in a new 3D in vitro model
Introduction: Extensive investigation has been undertaken regarding drug delivery systems for the management of glioblastoma multiforme (GBM). The infiltrative behavior of GBM cells within the brain tissue is primarily attributed to their heterogeneity, the movement of interstitial fluid (IFF), and...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Drug Delivery |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fddev.2023.1227776/full |
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| author | Wiam El Kheir Wiam El Kheir Anaïs Dumais Maude Beaudoin Bernard Marcos Nick Virgilio Benoit Paquette Benoit Paquette Nathalie Faucheux Nathalie Faucheux Marc-Antoine Lauzon Marc-Antoine Lauzon |
| author_facet | Wiam El Kheir Wiam El Kheir Anaïs Dumais Maude Beaudoin Bernard Marcos Nick Virgilio Benoit Paquette Benoit Paquette Nathalie Faucheux Nathalie Faucheux Marc-Antoine Lauzon Marc-Antoine Lauzon |
| author_sort | Wiam El Kheir |
| collection | DOAJ |
| description | Introduction: Extensive investigation has been undertaken regarding drug delivery systems for the management of glioblastoma multiforme (GBM). The infiltrative behavior of GBM cells within the brain tissue is primarily attributed to their heterogeneity, the movement of interstitial fluid (IFF), and the presence of chemokines. These factors contribute to the limited effectiveness of current conventional treatments. To address the dissemination of GBM cells, a proposed therapeutic approach involves utilizing a controlled release gradient of CXC-chemokine-ligand-12 (CXCL12). However, the impact of IFF on GBM cell migration within the brain underscores its critical importance as a significant parameter, which, surprisingly, has not been extensively studied in the context of localized drug delivery targeting the brain.Methods: Hydrogels are known for their inherent capacity to entrap various agents and exert precise control over their subsequent release. In the present investigation, we aimed to elucidate the release kinetics of CXCL12, whether in its free form or encapsulated within nanoparticles, from alginate-based hydrogels, both under static and dynamic conditions. To investigate the impact of convective forces mimicking the interstitial fluid flow (IFF) within the peritumoral environment of the brain, a three-dimensional in vitro model was developed. This model enabled the evaluation of CXCL12 release as a function of time and position, specifically accounting for the contribution of simulated IFF on the release behavior.Results: We first demonstrated that the release kinetic profiles under static culture conditions were independent of the initial mass loading and the predominant phenomenon occurring was diffusion. Subsequently, we investigated the release of CXCL12, which was loaded into Alginate/Chitosan-Nanoparticles (Alg/Chit-NPs) and embedded within an alginate hydrogel matrix. Mathematical modeling results also indicated the presence of electrostatic interactions between alginate and CXCL12. The Alg/Chit-NPs effectively slowed down the initial burst release, leading to a reduction in the diffusion coefficient of CXCL12. To further study the release behavior, we developed a perfusion bioreactor with a unique culture chamber designed to recapitulate the peritumoral environment and varied the fluid flow rates at 0.5 µL/min, 3 µL/min, 6.5 µL/min, and 10 µL/min. As the flow rate increased, the cumulative amount of released CXCL12 also increased for all three initial mass loadings. Beyond 3 µL/min, convection became the dominant mechanism governing CXCL12 release, whereas below this threshold, diffusion played a more prominent role.Conclusion: The indirect perfusion flow had a crucial impact on CXCL12 release and distribution inside the hydrogel in and against its direction. This system highlights the importance of considering the IFF in brain targeting delivery system and will be used in the future to study GBM cell behaviors in response to CXCL12 gradient. |
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| language | English |
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| publishDate | 2023-07-01 |
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| spelling | doaj.art-6f45eadd3dd4442580a22e24c78dba042024-08-03T05:44:40ZengFrontiers Media S.A.Frontiers in Drug Delivery2674-08502023-07-01310.3389/fddev.2023.12277761227776Impact of simulated brain interstitial fluid flow on the chemokine CXCL12 release from an alginate-based hydrogel in a new 3D in vitro modelWiam El Kheir0Wiam El Kheir1Anaïs Dumais2Maude Beaudoin3Bernard Marcos4Nick Virgilio5Benoit Paquette6Benoit Paquette7Nathalie Faucheux8Nathalie Faucheux9Marc-Antoine Lauzon10Marc-Antoine Lauzon11Advanced Dynamic Cell Culture Systems Laboratory, Department of Chemical and Biotechnological Engineering, Faculty of Engineering, Université de Sherbrooke, Sherbrooke, QC, CanadaLaboratory of Cell-Biomaterial Biohybrid Systems, Department of Chemical and Biotechnological Engineering, Faculty of Engineering, Université de Sherbrooke, Sherbrooke, QC, CanadaAdvanced Dynamic Cell Culture Systems Laboratory, Department of Chemical and Biotechnological Engineering, Faculty of Engineering, Université de Sherbrooke, Sherbrooke, QC, CanadaAdvanced Dynamic Cell Culture Systems Laboratory, Department of Chemical and Biotechnological Engineering, Faculty of Engineering, Université de Sherbrooke, Sherbrooke, QC, CanadaDepartment of Chemical Engineering and Biotechnological Engineering, Faculty of Engineering, Université de Sherbrooke, Sherbrooke, QC, CanadaDepartment of Chemical Engineering, Polytechnique Montréal, Montréal, QC, CanadaDepartment of Nuclear Medicine and Radiobiology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC, CanadaClinical Research Center of the Centre Hospitalier Universitaire de l’Université de Sherbrooke, Sherbrooke, QC, CanadaLaboratory of Cell-Biomaterial Biohybrid Systems, Department of Chemical and Biotechnological Engineering, Faculty of Engineering, Université de Sherbrooke, Sherbrooke, QC, CanadaClinical Research Center of the Centre Hospitalier Universitaire de l’Université de Sherbrooke, Sherbrooke, QC, CanadaAdvanced Dynamic Cell Culture Systems Laboratory, Department of Chemical and Biotechnological Engineering, Faculty of Engineering, Université de Sherbrooke, Sherbrooke, QC, CanadaResearch Center on Aging, Sherbrooke, QC, CanadaIntroduction: Extensive investigation has been undertaken regarding drug delivery systems for the management of glioblastoma multiforme (GBM). The infiltrative behavior of GBM cells within the brain tissue is primarily attributed to their heterogeneity, the movement of interstitial fluid (IFF), and the presence of chemokines. These factors contribute to the limited effectiveness of current conventional treatments. To address the dissemination of GBM cells, a proposed therapeutic approach involves utilizing a controlled release gradient of CXC-chemokine-ligand-12 (CXCL12). However, the impact of IFF on GBM cell migration within the brain underscores its critical importance as a significant parameter, which, surprisingly, has not been extensively studied in the context of localized drug delivery targeting the brain.Methods: Hydrogels are known for their inherent capacity to entrap various agents and exert precise control over their subsequent release. In the present investigation, we aimed to elucidate the release kinetics of CXCL12, whether in its free form or encapsulated within nanoparticles, from alginate-based hydrogels, both under static and dynamic conditions. To investigate the impact of convective forces mimicking the interstitial fluid flow (IFF) within the peritumoral environment of the brain, a three-dimensional in vitro model was developed. This model enabled the evaluation of CXCL12 release as a function of time and position, specifically accounting for the contribution of simulated IFF on the release behavior.Results: We first demonstrated that the release kinetic profiles under static culture conditions were independent of the initial mass loading and the predominant phenomenon occurring was diffusion. Subsequently, we investigated the release of CXCL12, which was loaded into Alginate/Chitosan-Nanoparticles (Alg/Chit-NPs) and embedded within an alginate hydrogel matrix. Mathematical modeling results also indicated the presence of electrostatic interactions between alginate and CXCL12. The Alg/Chit-NPs effectively slowed down the initial burst release, leading to a reduction in the diffusion coefficient of CXCL12. To further study the release behavior, we developed a perfusion bioreactor with a unique culture chamber designed to recapitulate the peritumoral environment and varied the fluid flow rates at 0.5 µL/min, 3 µL/min, 6.5 µL/min, and 10 µL/min. As the flow rate increased, the cumulative amount of released CXCL12 also increased for all three initial mass loadings. Beyond 3 µL/min, convection became the dominant mechanism governing CXCL12 release, whereas below this threshold, diffusion played a more prominent role.Conclusion: The indirect perfusion flow had a crucial impact on CXCL12 release and distribution inside the hydrogel in and against its direction. This system highlights the importance of considering the IFF in brain targeting delivery system and will be used in the future to study GBM cell behaviors in response to CXCL12 gradient.https://www.frontiersin.org/articles/10.3389/fddev.2023.1227776/fullglioblastoma multiformenanoparticleschemokines gradientkinetic releasemathematical modeling3D delivery system |
| spellingShingle | Wiam El Kheir Wiam El Kheir Anaïs Dumais Maude Beaudoin Bernard Marcos Nick Virgilio Benoit Paquette Benoit Paquette Nathalie Faucheux Nathalie Faucheux Marc-Antoine Lauzon Marc-Antoine Lauzon Impact of simulated brain interstitial fluid flow on the chemokine CXCL12 release from an alginate-based hydrogel in a new 3D in vitro model Frontiers in Drug Delivery glioblastoma multiforme nanoparticles chemokines gradient kinetic release mathematical modeling 3D delivery system |
| title | Impact of simulated brain interstitial fluid flow on the chemokine CXCL12 release from an alginate-based hydrogel in a new 3D in vitro model |
| title_full | Impact of simulated brain interstitial fluid flow on the chemokine CXCL12 release from an alginate-based hydrogel in a new 3D in vitro model |
| title_fullStr | Impact of simulated brain interstitial fluid flow on the chemokine CXCL12 release from an alginate-based hydrogel in a new 3D in vitro model |
| title_full_unstemmed | Impact of simulated brain interstitial fluid flow on the chemokine CXCL12 release from an alginate-based hydrogel in a new 3D in vitro model |
| title_short | Impact of simulated brain interstitial fluid flow on the chemokine CXCL12 release from an alginate-based hydrogel in a new 3D in vitro model |
| title_sort | impact of simulated brain interstitial fluid flow on the chemokine cxcl12 release from an alginate based hydrogel in a new 3d in vitro model |
| topic | glioblastoma multiforme nanoparticles chemokines gradient kinetic release mathematical modeling 3D delivery system |
| url | https://www.frontiersin.org/articles/10.3389/fddev.2023.1227776/full |
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