Cas9-guide RNA ribonucleoprotein-induced genome editing in the industrial green alga Coccomyxa sp. strain KJ

Cas9-guide RNA ribonucleoprotein-induced genome editing in the industrial green alga Coccomyxa sp. strain KJ

Abstract Background Oxygen-evolving photosynthetic microorganisms, collectively termed as microalgae, are gaining attention as alternative fuel sources. The unicellular alga Coccomyxa sp. strain KJ that belongs to the class Trebouxiophyceae can grow rapidly in minimal mineral media and accumulate tr...

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Bibliographic Details
Main Authors: Yuya Yoshimitsu, Jun Abe, Shigeaki Harayama
Format: Article
Language:English
Published: BMC 2018-12-01
Series:Biotechnology for Biofuels
Subjects:
Microalgae
Coccomyxa
Genome editing
Cas9-gRNA RNP
Electroporation
Online Access:http://link.springer.com/article/10.1186/s13068-018-1327-1

Internet

http://link.springer.com/article/10.1186/s13068-018-1327-1

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