Phosphorylation provides a negative mode of regulation for the yeast Rab GTPase Sec4p.
The Rab family of Ras-related GTPases are part of a complex signaling circuitry in eukaryotic cells, yet we understand little about the mechanisms that underlie Rab protein participation in such signal transduction networks, or how these networks are integrated at the physiological level. Reversible...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3171412?pdf=render |
| _version_ | 1818523227516829696 |
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| author | Christopher D Heger Christiane D Wrann Ruth N Collins |
| author_facet | Christopher D Heger Christiane D Wrann Ruth N Collins |
| author_sort | Christopher D Heger |
| collection | DOAJ |
| description | The Rab family of Ras-related GTPases are part of a complex signaling circuitry in eukaryotic cells, yet we understand little about the mechanisms that underlie Rab protein participation in such signal transduction networks, or how these networks are integrated at the physiological level. Reversible protein phosphorylation is widely used by cells as a signaling mechanism. Several phospho-Rabs have been identified, however the functional consequences of the modification appear to be diverse and need to be evaluated on an individual basis. In this study we demonstrate a role for phosphorylation as a negative regulatory event for the action of the yeast Rab GTPase Sec4p in regulating polarized growth. Our data suggest that the phosphorylation of the Rab Sec4p prevents interactions with its effector, the exocyst component Sec15p, and that the inhibition may be relieved by a PP2A phosphatase complex containing the regulatory subunit Cdc55p. |
| first_indexed | 2024-12-11T05:42:31Z |
| format | Article |
| id | doaj.art-6f6f20a483de47ce890c723dfb46877c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T05:42:31Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6f6f20a483de47ce890c723dfb46877c2022-12-22T01:19:05ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0169e2433210.1371/journal.pone.0024332Phosphorylation provides a negative mode of regulation for the yeast Rab GTPase Sec4p.Christopher D HegerChristiane D WrannRuth N CollinsThe Rab family of Ras-related GTPases are part of a complex signaling circuitry in eukaryotic cells, yet we understand little about the mechanisms that underlie Rab protein participation in such signal transduction networks, or how these networks are integrated at the physiological level. Reversible protein phosphorylation is widely used by cells as a signaling mechanism. Several phospho-Rabs have been identified, however the functional consequences of the modification appear to be diverse and need to be evaluated on an individual basis. In this study we demonstrate a role for phosphorylation as a negative regulatory event for the action of the yeast Rab GTPase Sec4p in regulating polarized growth. Our data suggest that the phosphorylation of the Rab Sec4p prevents interactions with its effector, the exocyst component Sec15p, and that the inhibition may be relieved by a PP2A phosphatase complex containing the regulatory subunit Cdc55p.http://europepmc.org/articles/PMC3171412?pdf=render |
| spellingShingle | Christopher D Heger Christiane D Wrann Ruth N Collins Phosphorylation provides a negative mode of regulation for the yeast Rab GTPase Sec4p. PLoS ONE |
| title | Phosphorylation provides a negative mode of regulation for the yeast Rab GTPase Sec4p. |
| title_full | Phosphorylation provides a negative mode of regulation for the yeast Rab GTPase Sec4p. |
| title_fullStr | Phosphorylation provides a negative mode of regulation for the yeast Rab GTPase Sec4p. |
| title_full_unstemmed | Phosphorylation provides a negative mode of regulation for the yeast Rab GTPase Sec4p. |
| title_short | Phosphorylation provides a negative mode of regulation for the yeast Rab GTPase Sec4p. |
| title_sort | phosphorylation provides a negative mode of regulation for the yeast rab gtpase sec4p |
| url | http://europepmc.org/articles/PMC3171412?pdf=render |
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