Three Molecular Modification Strategies to Improve the Thermostability of Xylanase XynA from <i>Streptomyces rameus</i> L2001

Three Molecular Modification Strategies to Improve the Thermostability of Xylanase XynA from <i>Streptomyces rameus</i> L2001

Glycoside hydrolase family 11 (GH11) xylanases are the preferred candidates for the production of functional oligosaccharides. However, the low thermostability of natural GH11 xylanases limits their industrial applications. In this study, we investigated the following three strategies to modify the...

Full description

Bibliographic Details
Main Authors:	Weijia Zhu, Liqin Qin, Youqiang Xu, Hongyun Lu, Qiuhua Wu, Weiwei Li, Chengnan Zhang, Xiuting Li
Format:	Article
Language:	English
Published:	MDPI AG 2023-02-01
Series:	Foods
Subjects:	GH11 xylanase thermostability surface entropy disulfide bond molecular cyclization
Online Access:	https://www.mdpi.com/2304-8158/12/4/879

Similar Items

Improving Thermostability of GH11 Xylanase XynASP by the Design of Loop Region
by: Tongbiao Li, et al.
Published: (2022-08-01)

Improvement of thermostability and catalytic efficiency of xylanase from Myceliophthora thermophilar by N-terminal and C-terminal truncation
by: Yue Yang, et al.
Published: (2024-04-01)

XynDZ5: A New Thermostable GH10 Xylanase
by: Dimitra Zarafeta, et al.
Published: (2020-04-01)

Cloning and expression of recombinant xylanase enzyme (xynA) in E. coli.
by: Amin Sadeghi Alikelayeh, et al.
Published: (2021-01-01)

Mutagenesis of N-terminal residues confer thermostability on a Penicillium janthinellum MA21601 xylanase
by: Ke Xiong, et al.
Published: (2019-07-01)

Enzymatic Characterization of Xylanase TgXyn2
by: Yuhang GE, et al.
Published: (2022-02-01)

Improvement in catalytic activity and thermostability of a GH10 xylanase and its synergistic degradation of biomass with cellulase
by: Shuai You, et al.
Published: (2019-12-01)

Production of Xylooligosaccharides from <i>Jiuzao</i> by Autohydrolysis Coupled with Enzymatic Hydrolysis Using a Thermostable Xylanase
by: Liqin Qin, et al.
Published: (2022-09-01)

Glycosyl hydrolase 11 (xynA) gene with xylanase activity from thermophilic bacteria isolated from thermal springs
by: Johnson Beslin Joshi, et al.
Published: (2022-04-01)

Improving the Thermostability of a Fungal GH11 Xylanase via Fusion of a Submodule (C2) from Hyperthermophilic CBM9_1-2
by: Huabiao Miao, et al.
Published: (2021-12-01)

Production, Purification, and Characterization of Thermostable Alkaline Xylanase From Anoxybacillus kamchatkensis NASTPD13
by: Punam Yadav, et al.
Published: (2018-05-01)

A novel thermostable GH10 xylanase with activities on a wide variety of cellulosic substrates from a xylanolytic Bacillus strain exhibiting significant synergy with commercial Celluclast 1.5 L in pretreated corn stover hydrolysis
by: Kui Wang, et al.
Published: (2019-03-01)

Thermostable Xylanase Production by Geobacillus sp. Strain DUSELR13, and Its Application in Ethanol Production with Lignocellulosic Biomass
by: Mohit Bibra, et al.
Published: (2018-09-01)

THE EFFECT OF ALCOHOLS, LIGNIN AND PHENOLIC COMPOUNDS ON THE ENZYME ACTIVITY OF CLOSTRIDIUM CELLULOVORANS XynA
by: David Morrison, et al.
Published: (2011-07-01)

Effects of substrate binding site residue substitutions of xynA from Bacillus amyloliquefaciens on substrate specificity
by: Anil S. Prajapati, et al.
Published: (2018-02-01)

Partial purification and characterization of a thermostable xylanase from Bacillus licheniformis isolated from hot water geyser
by: Girisha Malhotra, et al.
Published: (2022-03-01)

Investigation of a thermostable multi-domain xylanase-glucuronoyl esterase enzyme from Caldicellulosiruptor kristjanssonii incorporating multiple carbohydrate-binding modules
by: Daniel Krska, et al.
Published: (2020-04-01)

Highly thermostable xylanase production from a thermophilic Geobacillus sp. strain WSUCF1 utilizing lignocellulosic biomass
by: Aditya eBhalla, et al.
Published: (2015-06-01)

CaXyn30B from the solventogenic bacterium Clostridium acetobutylicum is a glucuronic acid-dependent endoxylanase
by: Casey Crooks, et al.
Published: (2020-06-01)

Optimization of biosynthesis conditions and catalitic behavior evaluation of cellulase-free xylanase produced by a new Streptomyces sp. strain
by: GABRIELA BAHRIM, et al.
Published: (2011-07-01)

Effective synthesis of circRNA via a thermostable T7 RNA polymerase variant as the catalyst
by: Wei He, et al.
Published: (2024-04-01)

Improving the thermostability of a fungal GH11 xylanase via site-directed mutagenesis guided by sequence and structural analysis
by: Nanyu Han, et al.
Published: (2017-05-01)

GH10 xylanase D from <it>Penicillium funiculosum</it>: biochemical studies and xylooligosaccharide production
by: Giardina Thierry, et al.
Published: (2011-04-01)

A Comparative Study to Decipher the Structural and Dynamics Determinants Underlying the Activity and Thermal Stability of GH-11 Xylanases
by: Jelena Vucinic, et al.
Published: (2021-05-01)

Disulfide bond engineering of AppA phytase for increased thermostability requires co-expression of protein disulfide isomerase in Pichia pastoris
by: Laura Navone, et al.
Published: (2021-03-01)

Immobilization of mutated xylanase from Neocallimastix patriciarum in E. coli and application for kraft pulp biobleaching
by: Y. Huang, et al.
Published: (2021-08-01)

Effects of Site-Directed Mutations on the Communicability between Local Segments and Binding Pocket Distortion of Engineered GH11 Xylanases Visualized through Network Topology Analysis
by: Thana Sutthibutpong, et al.
Published: (2022-10-01)

Thermostability improvement of xylanase using error-prone polymerase chain reaction /
by: Mohd. Khairul Hakimi Abdul Wahab, et al.
Published: (2013)

A novel fungal GH30 xylanase with xylobiohydrolase auxiliary activity
by: Constantinos Katsimpouras, et al.
Published: (2019-05-01)

Disulfide Bond Engineering for Enhancing the Thermostability of the Maltotetraose-Forming Amylase from <i>Pseudomonas saccharophila</i> STB07
by: Yinglan Wang, et al.
Published: (2022-04-01)

Engineering of GH11 Xylanases for Optimal pH Shifting for Industrial Applications
by: In Jung Kim, et al.
Published: (2023-10-01)

Improving the thermostability of GH49 dextranase AoDex by site-directed mutagenesis
by: Zhen Wei, et al.
Published: (2023-01-01)

Purification and Characterization of a Xylanase from the Newly Isolated Penicillium rolfsii c3-2(1) IBRL
by: Kok Chang Lee, et al.
Published: (2015-01-01)

The Xylanase Inhibitor TAXI-I Increases Plant Resistance to <i>Botrytis cinerea</i> by Inhibiting the BcXyn11a Xylanase Necrotizing Activity
by: Silvio Tundo, et al.
Published: (2020-05-01)

Thermostability improvement of xylanase using error-prone polymerase chain reaction [electronic resource] /
by: 556236 Mohd. Khairul Hakimi Abdul Wahab
Published: (2013)

Improving the thermostability of Trichoderma reesei xylanase 2 by introducing disulfide bonds
by: Feng Tang, et al.
Published: (2017-03-01)

Optimization of fermentation strategy for enhanced production of thermostable xylanase by recombinant Escherichia Coli
by: Kandiyil, Subeesh Kunhi
Published: (2023)

Structural Insight Into Chitin Degradation and Thermostability of a Novel Endochitinase From the Glycoside Hydrolase Family 18
by: Yan-Jun Wang, et al.
Published: (2019-10-01)

Determination of the Nucleotide Sequence of a Thermostable Xylanase Gene from Bacillus Coagulans ST-6
by: M. S. Suhaimi, Ainu Husna
Published: (1999)

A 25-Residue Peptide From Botrytis cinerea Xylanase BcXyn11A Elicits Plant Defenses
by: Marcos Frías, et al.
Published: (2019-04-01)