Crocin mitigates atherosclerotic progression in LDLR knockout mice by hepatic oxidative stress and inflammatory reaction reduction, and intestinal barrier improvement and gut microbiota modulation
Crocin has a protective effect on neurodegenerative disease, but whether crocin can prevent atherosclerosis remains unclear. The present study aimed to explore the possible anti-atherosclerotic mechanism of crocin in LDLR-/- mice. Mice were fed an atherogenic high-fat/cholesterol diet and concomitan...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464622002912 |
| _version_ | 1818009658735984640 |
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| author | Shufen Han Ruijuan Song Yifei Cao Xiao Yan Hui Gao Fuzhi Lian |
| author_facet | Shufen Han Ruijuan Song Yifei Cao Xiao Yan Hui Gao Fuzhi Lian |
| author_sort | Shufen Han |
| collection | DOAJ |
| description | Crocin has a protective effect on neurodegenerative disease, but whether crocin can prevent atherosclerosis remains unclear. The present study aimed to explore the possible anti-atherosclerotic mechanism of crocin in LDLR-/- mice. Mice were fed an atherogenic high-fat/cholesterol diet and concomitantly treated by intragastric administration with normal saline (Model) or 20 or 40 mg/(kg.bw.day) crocin for 8 weeks. Pathological results showed that Model group exhibited a marked atherosclerotic plaque, while crocin significantly reduced atherosclerotic plaque, accompanied by a small amount of denatured collagen fiber and foam cells. Mechanistically, crocin, especially high-dose suppressed the formation of atherosclerotic plaques via enhancing hepatic antioxidant capacity. Besides, crocin effectively inhibited NLRP3 inflammasome and blocked TLR4 signal, through reducing oxidative stress based on Nrf2/Keap1 pathway, and increasing intestinal tight junction proteins and improving gut microbiota composition. These results suggest that crocin can ameliorate inflammation and attenuate atherosclerotic progression by oxidative stress reduction and intestinal barrier improvement. |
| first_indexed | 2024-04-14T05:45:49Z |
| format | Article |
| id | doaj.art-6fa0fd22d9d04496bc22076403bd55de |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-04-14T05:45:49Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-6fa0fd22d9d04496bc22076403bd55de2022-12-22T02:09:18ZengElsevierJournal of Functional Foods1756-46462022-09-0196105221Crocin mitigates atherosclerotic progression in LDLR knockout mice by hepatic oxidative stress and inflammatory reaction reduction, and intestinal barrier improvement and gut microbiota modulationShufen Han0Ruijuan Song1Yifei Cao2Xiao Yan3Hui Gao4Fuzhi Lian5Department of Nutrition and Toxicology, School of Public Health, Hangzhou Normal University, Hangzhou 311121, Zhejiang, PR China; Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou 215123, Jiangsu, PR China; Corresponding authors at: Department of Nutrition and Toxicology, School of Public Health, Hangzhou Normal University, 2318 Yuhangtang Road, Hangzhou 311121, Zhejiang, China (S. Han). Department of Nutrition and Toxicology, School of Public Health, Hangzhou Normal University, 2318 Yuhangtang Road, Hangzhou 311121, Zhejiang, China (F. Lian).Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou 215123, Jiangsu, PR ChinaDepartment of Nutrition and Toxicology, School of Public Health, Hangzhou Normal University, Hangzhou 311121, Zhejiang, PR ChinaDepartment of Nutrition and Toxicology, School of Public Health, Hangzhou Normal University, Hangzhou 311121, Zhejiang, PR ChinaDepartment of Nutrition and Food Hygiene, School of Public Health, Soochow University, Suzhou 215123, Jiangsu, PR ChinaDepartment of Nutrition and Toxicology, School of Public Health, Hangzhou Normal University, Hangzhou 311121, Zhejiang, PR China; Corresponding authors at: Department of Nutrition and Toxicology, School of Public Health, Hangzhou Normal University, 2318 Yuhangtang Road, Hangzhou 311121, Zhejiang, China (S. Han). Department of Nutrition and Toxicology, School of Public Health, Hangzhou Normal University, 2318 Yuhangtang Road, Hangzhou 311121, Zhejiang, China (F. Lian).Crocin has a protective effect on neurodegenerative disease, but whether crocin can prevent atherosclerosis remains unclear. The present study aimed to explore the possible anti-atherosclerotic mechanism of crocin in LDLR-/- mice. Mice were fed an atherogenic high-fat/cholesterol diet and concomitantly treated by intragastric administration with normal saline (Model) or 20 or 40 mg/(kg.bw.day) crocin for 8 weeks. Pathological results showed that Model group exhibited a marked atherosclerotic plaque, while crocin significantly reduced atherosclerotic plaque, accompanied by a small amount of denatured collagen fiber and foam cells. Mechanistically, crocin, especially high-dose suppressed the formation of atherosclerotic plaques via enhancing hepatic antioxidant capacity. Besides, crocin effectively inhibited NLRP3 inflammasome and blocked TLR4 signal, through reducing oxidative stress based on Nrf2/Keap1 pathway, and increasing intestinal tight junction proteins and improving gut microbiota composition. These results suggest that crocin can ameliorate inflammation and attenuate atherosclerotic progression by oxidative stress reduction and intestinal barrier improvement.http://www.sciencedirect.com/science/article/pii/S1756464622002912CrocinOxidative stressInflammationIntestinal barrierGut microbiotaAtherosclerosis |
| spellingShingle | Shufen Han Ruijuan Song Yifei Cao Xiao Yan Hui Gao Fuzhi Lian Crocin mitigates atherosclerotic progression in LDLR knockout mice by hepatic oxidative stress and inflammatory reaction reduction, and intestinal barrier improvement and gut microbiota modulation Journal of Functional Foods Crocin Oxidative stress Inflammation Intestinal barrier Gut microbiota Atherosclerosis |
| title | Crocin mitigates atherosclerotic progression in LDLR knockout mice by hepatic oxidative stress and inflammatory reaction reduction, and intestinal barrier improvement and gut microbiota modulation |
| title_full | Crocin mitigates atherosclerotic progression in LDLR knockout mice by hepatic oxidative stress and inflammatory reaction reduction, and intestinal barrier improvement and gut microbiota modulation |
| title_fullStr | Crocin mitigates atherosclerotic progression in LDLR knockout mice by hepatic oxidative stress and inflammatory reaction reduction, and intestinal barrier improvement and gut microbiota modulation |
| title_full_unstemmed | Crocin mitigates atherosclerotic progression in LDLR knockout mice by hepatic oxidative stress and inflammatory reaction reduction, and intestinal barrier improvement and gut microbiota modulation |
| title_short | Crocin mitigates atherosclerotic progression in LDLR knockout mice by hepatic oxidative stress and inflammatory reaction reduction, and intestinal barrier improvement and gut microbiota modulation |
| title_sort | crocin mitigates atherosclerotic progression in ldlr knockout mice by hepatic oxidative stress and inflammatory reaction reduction and intestinal barrier improvement and gut microbiota modulation |
| topic | Crocin Oxidative stress Inflammation Intestinal barrier Gut microbiota Atherosclerosis |
| url | http://www.sciencedirect.com/science/article/pii/S1756464622002912 |
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