Long-read sequence analysis of MMEJ-mediated CRISPR genome editing reveals complex on-target vector insertions that may escape standard PCR-based quality control
Abstract CRISPR genome editing is a powerful tool for elucidating biological functions. To modify the genome as intended, it is essential to understand the various modes of recombination that can occur. In this study, we report complex vector insertions that were identified during the generation of...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2023-07-01
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Series: | Scientific Reports |
Online Access: | https://doi.org/10.1038/s41598-023-38397-y |