TAMRA/TAMRA Fluorescence Quenching Systems for the Activity Assay of Alkaline Phosphatase

TAMRA/TAMRA Fluorescence Quenching Systems for the Activity Assay of Alkaline Phosphatase

We introduce two types of fluorescence-quenching assay for alkaline phosphatases (APs) by using a carboxytetramethyl-rhodamine (TAMRA)-labeled phosphate-binding tag molecule (TAMRA-Phos-tag). In the first assay, TAMRA-labeled O-phosphorylethanolamine (TAMRA-PEA) was used as an artificial AP-substrat...

Full description

Bibliographic Details
Main Authors: Akio Shiba, Emiko Kinoshita-Kikuta, Eiji Kinoshita, Tohru Koike
Format: Article
Language:English
Published: MDPI AG 2017-08-01
Series:Sensors
Subjects:
alkaline phosphatase
fluorometric analysis
fluorescence quenching
tetramethylrhodamine
O-phosphorylethanolamine
pyrophosphate
Online Access:https://www.mdpi.com/1424-8220/17/8/1877

Internet

https://www.mdpi.com/1424-8220/17/8/1877

Similar Items