产油核桃内生细菌乙酰辅酶A羧化酶acb基因 克隆及优化表达Cloning and expression optimization of acetyl-CoA carboxylase acb gene from oil-producing walnut endophyte

产油核桃内生细菌乙酰辅酶A羧化酶acb基因 克隆及优化表达Cloning and expression optimization of acetyl-CoA carboxylase acb gene from oil-producing walnut endophyte

为了探究细菌异质性乙酰辅酶A羧化酶β亚基生物活性及其对乙酰辅酶A羧化酶酶活影响，以高产油核桃内生细菌B. subtilis HB1310基因组DNA为模板，利用PCR技术扩增其乙酰辅酶A羧化酶羧基转移酶β亚基（β-CT）基因（acb基因），构建pET-28a-acb载体并在E. coli BL21中表达，进一步对乙酰辅酶A羧化酶acb基因的诱导表达条件进行了优化。结果表明：乙酰辅酶A羧化酶acb基因在E. coli BL21中实现了表达，分子质量在25~35 kDa之间；重组蛋白最佳诱导表达条件为诱导剂异丙基-β-D-硫代半乳糖苷（IPTG）浓度1.0 mmol/L、诱导时间6 h、诱导初始p...

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Bibliographic Details
Main Author: 许思远，张永贵，赵沛，曹娟娟，张琴XU Siyuan，ZHANG Yonggui，ZHAO Pei，CAO Juanjuan，ZHANG Qin
Format: Article
Language:English
Published: 中粮工科（西安）国际工程有限公司 2023-04-01
Series:Zhongguo youzhi
Subjects:
核桃内生细菌；产油；乙酰辅酶a羧化酶；克隆；优化表达
walnut endophyte; oil-producing; acetyl-coa carboxylase; cloning; expression optimization
Online Access:http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20230418&year_id=2023&quarter_id=4&falg=1

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http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20230418&year_id=2023&quarter_id=4&falg=1

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