产油核桃内生细菌乙酰辅酶A羧化酶acb基因 克隆及优化表达Cloning and expression optimization of acetyl-CoA carboxylase acb gene from oil-producing walnut endophyte
为了探究细菌异质性乙酰辅酶A羧化酶β亚基生物活性及其对乙酰辅酶A羧化酶酶活影响,以高产油核桃内生细菌B. subtilis HB1310基因组DNA为模板,利用PCR技术扩增其乙酰辅酶A羧化酶羧基转移酶β亚基(β-CT)基因(acb基因),构建pET-28a-acb载体并在E. coli BL21中表达,进一步对乙酰辅酶A羧化酶acb基因的诱导表达条件进行了优化。结果表明:乙酰辅酶A羧化酶acb基因在E. coli BL21中实现了表达,分子质量在25~35 kDa之间;重组蛋白最佳诱导表达条件为诱导剂异丙基-β-D-硫代半乳糖苷(IPTG)浓度1.0 mmol/L、诱导时间6 h、诱导初始p...
Main Author: | 许思远,张永贵,赵沛,曹娟娟,张琴XU Siyuan,ZHANG Yonggui,ZHAO Pei,CAO Juanjuan,ZHANG Qin |
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Format: | Article |
Language: | English |
Published: |
中粮工科(西安)国际工程有限公司
2023-04-01
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Series: | Zhongguo youzhi |
Subjects: | |
Online Access: | http://tg.chinaoils.cn/ch/reader/create_pdf.aspx?file_no=20230418&year_id=2023&quarter_id=4&falg=1 |
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