High-throughput monitoring of integration site clonality in preclinical and clinical gene therapy studies

High-throughput monitoring of integration site clonality in preclinical and clinical gene therapy studies

Gene transfer to hematopoietic stem cells with integrating vectors not only allows sustained correction of monogenic diseases but also tracking of individual clones in vivo. Quantitative real-time PCR (qPCR) has been shown to be an accurate method to quantify individual stem cell clones, yet due to...

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Bibliographic Details
Main Authors: Frank A Giordano, Jens-Uwe Appelt, Barbara Link, Sebastian Gerdes, Christina Lehrer, Simone Scholz, Anna Paruzynski, Ingo Roeder, Frederik Wenz, Hanno Glimm, Christof von Kalle, Manuel Grez, Manfred Schmidt, Stephanie Laufs
Format: Article
Language:English
Published: Elsevier 2015-01-01
Series:Molecular Therapy: Methods & Clinical Development
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050116300055

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http://www.sciencedirect.com/science/article/pii/S2329050116300055

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