Design and Validation of Plasmid Vectors for Characterizing Protein–Protein Interactions in Spodoptera frugiperda Insect Cells

Design and Validation of Plasmid Vectors for Characterizing Protein–Protein Interactions in Spodoptera frugiperda Insect Cells

There are limited molecular biology resources for interrogating protein–protein interactions (PPIs) in insect cells. To address this deficiency, we developed plasmid vectors for localization, bimolecular fluorescence complementation (BiFC), and co-immunoprecipitation (co-IP) assays in Sf9 insect cel...

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Bibliographic Details
Main Authors: Hao Wei Teh, Kathleen M. Martin, Anna E. Whitfield
Format: Article
Language:English
Published: The American Phytopathological Society 2022-06-01
Series:PhytoFrontiers
Subjects:
cell biology
host parasite interactions
insects
rhabdovirus
virology
Online Access:https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-09-21-0059-TA

Internet

https://apsjournals.apsnet.org/doi/10.1094/PHYTOFR-09-21-0059-TA

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