hilD Is Required for the Active Internalization of Salmonella Newport into Cherry Tomatoes
Salmonella enterica is a foodborne pathogen that can be internalized into fresh produce. Most of the Salmonella virulence genes are clustered in regions denominated Salmonella Pathogenicity Islands (SPI). SPI-1 encodes a Type Three Secretion System (T3SS-1) and effector proteins that allow the inter...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Journal of Food Protection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0362028X23067571 |
| _version_ | 1797788463177662464 |
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| author | Aurora Dolores Arista-Regalado Jeannette Barba-León Victor Humberto Bustamante Mario Alberto Flores-Valdez Jorge Gaona Maira Juliana Fajardo-Guerrero |
| author_facet | Aurora Dolores Arista-Regalado Jeannette Barba-León Victor Humberto Bustamante Mario Alberto Flores-Valdez Jorge Gaona Maira Juliana Fajardo-Guerrero |
| author_sort | Aurora Dolores Arista-Regalado |
| collection | DOAJ |
| description | Salmonella enterica is a foodborne pathogen that can be internalized into fresh produce. Most of the Salmonella virulence genes are clustered in regions denominated Salmonella Pathogenicity Islands (SPI). SPI-1 encodes a Type Three Secretion System (T3SS-1) and effector proteins that allow the internalization of Salmonella into animal cells. HilD is a transcriptional regulator that induces the expression of SPI-1 genes and other related virulence genes located outside of this island. Here, we assessed the role of hilD in the internalization of Salmonella Newport and Typhimurium into cherry tomatoes, by evaluating either an isolate from an avocado orchard, S. Newport-45 or the laboratory strain S. Typhimurium SL1344 and their isogenic mutants in hilD. The internalization of these bacteria was carried out by using a temperature gradient of 12°C. The transcription of hilD and invA was tested by qRT-PCR experiments. Our results show that S. Newport-45 hilD mutant viable cells obtained from the interior of the fruit were decreased (2.7-fold), compared with those observed for S. Typhimurium SL1344. Interestingly, at 3 days postinoculation, the cells recovered from S. Newport-45 hilD mutant were similar to those recovered from all the strains evaluated, suggesting that hilD is required only for the initial internalization of S. Newport. |
| first_indexed | 2024-03-13T01:36:54Z |
| format | Article |
| id | doaj.art-706de58acd5c49b9ba8c41c9c595e29b |
| institution | Directory Open Access Journal |
| issn | 0362-028X |
| language | English |
| last_indexed | 2024-03-13T01:36:54Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Food Protection |
| spelling | doaj.art-706de58acd5c49b9ba8c41c9c595e29b2023-07-04T05:08:10ZengElsevierJournal of Food Protection0362-028X2023-05-01865100085hilD Is Required for the Active Internalization of Salmonella Newport into Cherry TomatoesAurora Dolores Arista-Regalado0Jeannette Barba-León1Victor Humberto Bustamante2Mario Alberto Flores-Valdez3Jorge Gaona4Maira Juliana Fajardo-Guerrero5Microbiology and Pathology department Centro, Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco 44340, MexicoPublic Health department Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan, Jalisco 45200, MexicoMolecular Microbiology department Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62210, MexicoMedical and Pharmaceutical Biotechnology department Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. Guadalajara, Jalisco 44270, MexicoMicrobiology and Pathology department Centro, Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco 44340, MexicoPublic Health department Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Zapopan, Jalisco 45200, MexicoSalmonella enterica is a foodborne pathogen that can be internalized into fresh produce. Most of the Salmonella virulence genes are clustered in regions denominated Salmonella Pathogenicity Islands (SPI). SPI-1 encodes a Type Three Secretion System (T3SS-1) and effector proteins that allow the internalization of Salmonella into animal cells. HilD is a transcriptional regulator that induces the expression of SPI-1 genes and other related virulence genes located outside of this island. Here, we assessed the role of hilD in the internalization of Salmonella Newport and Typhimurium into cherry tomatoes, by evaluating either an isolate from an avocado orchard, S. Newport-45 or the laboratory strain S. Typhimurium SL1344 and their isogenic mutants in hilD. The internalization of these bacteria was carried out by using a temperature gradient of 12°C. The transcription of hilD and invA was tested by qRT-PCR experiments. Our results show that S. Newport-45 hilD mutant viable cells obtained from the interior of the fruit were decreased (2.7-fold), compared with those observed for S. Typhimurium SL1344. Interestingly, at 3 days postinoculation, the cells recovered from S. Newport-45 hilD mutant were similar to those recovered from all the strains evaluated, suggesting that hilD is required only for the initial internalization of S. Newport.http://www.sciencedirect.com/science/article/pii/S0362028X23067571Cherry tomatoeshilDInternalizationS. NewportS. TyphimuriumSalmonella |
| spellingShingle | Aurora Dolores Arista-Regalado Jeannette Barba-León Victor Humberto Bustamante Mario Alberto Flores-Valdez Jorge Gaona Maira Juliana Fajardo-Guerrero hilD Is Required for the Active Internalization of Salmonella Newport into Cherry Tomatoes Journal of Food Protection Cherry tomatoes hilD Internalization S. Newport S. Typhimurium Salmonella |
| title | hilD Is Required for the Active Internalization of Salmonella Newport into Cherry Tomatoes |
| title_full | hilD Is Required for the Active Internalization of Salmonella Newport into Cherry Tomatoes |
| title_fullStr | hilD Is Required for the Active Internalization of Salmonella Newport into Cherry Tomatoes |
| title_full_unstemmed | hilD Is Required for the Active Internalization of Salmonella Newport into Cherry Tomatoes |
| title_short | hilD Is Required for the Active Internalization of Salmonella Newport into Cherry Tomatoes |
| title_sort | hild is required for the active internalization of salmonella newport into cherry tomatoes |
| topic | Cherry tomatoes hilD Internalization S. Newport S. Typhimurium Salmonella |
| url | http://www.sciencedirect.com/science/article/pii/S0362028X23067571 |
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