The Effects of Optical Aberrations to Illumination Beam Thickness in Two-Photon Excitation Microscopes

When performing in vivo imaging of live samples, it is a big challenge to penetrate thick tissues while still maintaining high resolution and a large field of view because of the sample-induced aberrations. These requirements can be met by combining the benefits of two-photon excitation, beam modula...

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Bibliographic Details
Main Authors: Nan Li, Fanglin Luo, Chengliang Yang, Zenghui Peng, Li Xuan, Qingpan Bu, Quanquan Mu, Xingyun Zhang
Format: Article
Language:English
Published: MDPI AG 2022-07-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/12/14/7156