Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs

<p>Abstract</p> <p>MicroRNAs (miRNAs) are a class of small non-coding RNAs with a critical role in development and environmental responses. Efficient and reliable detection of miRNAs is an essential step towards understanding their roles in specific cells and tissues. However, gel-...

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Bibliographic Details
Main Authors: Walton Eric F, Wood Marion, Wu Rongmei, Varkonyi-Gasic Erika, Hellens Roger P
Format: Article
Language:English
Published: BMC 2007-10-01
Series:Plant Methods
Online Access:http://www.plantmethods.com/content/3/1/12