Determination of S- and R-Amlodipine in Rat Plasma using LC-MS/MS After Oral Administration of S-Amlodipine and Racemic Amlodipine
The pharmacokinetic properties of S-amlodipine were studied using racemic amlodipine and single S-enantiomer(SK310) administration to rats. Plasma levels of the drug were determined using chiral liquid chromatography coupled with tandemmass spectrometry following solid phase extraction. The stereosp...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Society for Mass Spectrometry
2011-12-01
|
| Series: | Mass Spectrometry Letters |
| Online Access: | http://Amlodipine, LC-MS/MS, Pharmacokinetics, Rat plasma |
| _version_ | 1811315283898400768 |
|---|---|
| author | Dong-Hyun Kim Tae Kon Kim Bong-Yong Lee Hye Hyun Yoo |
| author_facet | Dong-Hyun Kim Tae Kon Kim Bong-Yong Lee Hye Hyun Yoo |
| author_sort | Dong-Hyun Kim |
| collection | DOAJ |
| description | The pharmacokinetic properties of S-amlodipine were studied using racemic amlodipine and single S-enantiomer(SK310) administration to rats. Plasma levels of the drug were determined using chiral liquid chromatography coupled with tandemmass spectrometry following solid phase extraction. The stereospecific analysis of amlodipine was performed on an α-acidglycoprotein (AGP) column using a mobile phase comprising 10 mM ammonium acetate (pH 4.0) and propanol at a flow rate of0.2 mL/min. This method was used to perform a comparative study of the pharmacokinetics of amlodipine and SK310. Theresults revealed that the pharmacokinetic profile of S-amlodipine after the administration of SK310 was comparable to thatfollowing the administration of the racemic mixture. |
| first_indexed | 2024-04-13T11:27:36Z |
| format | Article |
| id | doaj.art-70d7c90bfbfe40118c884d9454b06510 |
| institution | Directory Open Access Journal |
| issn | 2233-4203 2093-8950 |
| language | English |
| last_indexed | 2024-04-13T11:27:36Z |
| publishDate | 2011-12-01 |
| publisher | Korean Society for Mass Spectrometry |
| record_format | Article |
| series | Mass Spectrometry Letters |
| spelling | doaj.art-70d7c90bfbfe40118c884d9454b065102022-12-22T02:48:39ZengKorean Society for Mass SpectrometryMass Spectrometry Letters2233-42032093-89502011-12-01248891Determination of S- and R-Amlodipine in Rat Plasma using LC-MS/MS After Oral Administration of S-Amlodipine and Racemic AmlodipineDong-Hyun KimTae Kon KimBong-Yong LeeHye Hyun YooThe pharmacokinetic properties of S-amlodipine were studied using racemic amlodipine and single S-enantiomer(SK310) administration to rats. Plasma levels of the drug were determined using chiral liquid chromatography coupled with tandemmass spectrometry following solid phase extraction. The stereospecific analysis of amlodipine was performed on an α-acidglycoprotein (AGP) column using a mobile phase comprising 10 mM ammonium acetate (pH 4.0) and propanol at a flow rate of0.2 mL/min. This method was used to perform a comparative study of the pharmacokinetics of amlodipine and SK310. Theresults revealed that the pharmacokinetic profile of S-amlodipine after the administration of SK310 was comparable to thatfollowing the administration of the racemic mixture.Amlodipine, LC-MS/MS, Pharmacokinetics, Rat plasma |
| spellingShingle | Dong-Hyun Kim Tae Kon Kim Bong-Yong Lee Hye Hyun Yoo Determination of S- and R-Amlodipine in Rat Plasma using LC-MS/MS After Oral Administration of S-Amlodipine and Racemic Amlodipine Mass Spectrometry Letters |
| title | Determination of S- and R-Amlodipine in Rat Plasma using LC-MS/MS After Oral Administration of S-Amlodipine and Racemic Amlodipine |
| title_full | Determination of S- and R-Amlodipine in Rat Plasma using LC-MS/MS After Oral Administration of S-Amlodipine and Racemic Amlodipine |
| title_fullStr | Determination of S- and R-Amlodipine in Rat Plasma using LC-MS/MS After Oral Administration of S-Amlodipine and Racemic Amlodipine |
| title_full_unstemmed | Determination of S- and R-Amlodipine in Rat Plasma using LC-MS/MS After Oral Administration of S-Amlodipine and Racemic Amlodipine |
| title_short | Determination of S- and R-Amlodipine in Rat Plasma using LC-MS/MS After Oral Administration of S-Amlodipine and Racemic Amlodipine |
| title_sort | determination of s and r amlodipine in rat plasma using lc ms ms after oral administration of s amlodipine and racemic amlodipine |
| url | http://Amlodipine, LC-MS/MS, Pharmacokinetics, Rat plasma |
| work_keys_str_mv | AT donghyunkim determinationofsandramlodipineinratplasmausinglcmsmsafteroraladministrationofsamlodipineandracemicamlodipine AT taekonkim determinationofsandramlodipineinratplasmausinglcmsmsafteroraladministrationofsamlodipineandracemicamlodipine AT bongyonglee determinationofsandramlodipineinratplasmausinglcmsmsafteroraladministrationofsamlodipineandracemicamlodipine AT hyehyunyoo determinationofsandramlodipineinratplasmausinglcmsmsafteroraladministrationofsamlodipineandracemicamlodipine |